Mehmet Sadik Akca, Omer Kemal Kinaci, Bulent Inanc
{"title":"流体动力学研究：通过涡流诱导振动提高微藻培养中的光照可用性并在赛道池塘中创造高频率的光-暗循环。","authors":"Mehmet Sadik Akca, Omer Kemal Kinaci, Bulent Inanc","doi":"10.1007/s00449-024-03074-5","DOIUrl":null,"url":null,"abstract":"<p><p>Limited light availability due to insufficient vertical mixing strongly reduces the applicability of raceway ponds (RWPs). To overcome this and create light-dark (L/D) cycles for enhanced biomass production through improved vertical mixing, vortex-induced vibration (VIV) system was implemented by the authors in a previous study to an existing pilot-scale RWP. In this study, experimental characterization of fluid dynamics for VIV-implemented RWP is carried out. Particle image velocimetry (PIV) technique is applied to visualize the flow. The extents of the vertical mixing due to VIV and the characteristics of L/D cycles were examined by tracking selected particles. Pond depth was hypothetically divided into three zones, namely dark, light Iimited and light saturated for detailed analysis of cell trajectories. It has been observed that VIV cylinder oscillation can efficiently facilitate the transfer of cells from light-limited to light-saturated zones. Among the cells that were tracked, 44% initially at dark zone entered the light-limited zone and 100% of initially at light-limited zone entered the light-saturated zone. 33% of all tracked cells experienced high-frequency L/D cycles with an average frequency of 35.69 s<sup>-1</sup> and 0.49 light fraction. The impact of VIV was not discernible in the deeper sections of the pond, due to constrained oscillation amplitudes. Our findings suggest that the approximately 20% increase in biomass production reported in our previous study can be attributed to the synergistic effects of enhanced L/D cycle frequencies and improved light availability resulting from the transfer of cells from dark to light-limited zones. To further enhance the effectiveness of VIV, design improvements were developed. It was concluded that light availability could be significantly improved with the presented method for more effective use of RWPs.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":"1863-1874"},"PeriodicalIF":3.5000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11438835/pdf/","citationCount":"0","resultStr":"{\"title\":\"Improving light availability and creating high-frequency light-dark cycles in raceway ponds through vortex-induced vibrations for microalgae cultivation: a fluid dynamic study.\",\"authors\":\"Mehmet Sadik Akca, Omer Kemal Kinaci, Bulent Inanc\",\"doi\":\"10.1007/s00449-024-03074-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Limited light availability due to insufficient vertical mixing strongly reduces the applicability of raceway ponds (RWPs). To overcome this and create light-dark (L/D) cycles for enhanced biomass production through improved vertical mixing, vortex-induced vibration (VIV) system was implemented by the authors in a previous study to an existing pilot-scale RWP. In this study, experimental characterization of fluid dynamics for VIV-implemented RWP is carried out. Particle image velocimetry (PIV) technique is applied to visualize the flow. The extents of the vertical mixing due to VIV and the characteristics of L/D cycles were examined by tracking selected particles. Pond depth was hypothetically divided into three zones, namely dark, light Iimited and light saturated for detailed analysis of cell trajectories. It has been observed that VIV cylinder oscillation can efficiently facilitate the transfer of cells from light-limited to light-saturated zones. Among the cells that were tracked, 44% initially at dark zone entered the light-limited zone and 100% of initially at light-limited zone entered the light-saturated zone. 33% of all tracked cells experienced high-frequency L/D cycles with an average frequency of 35.69 s<sup>-1</sup> and 0.49 light fraction. The impact of VIV was not discernible in the deeper sections of the pond, due to constrained oscillation amplitudes. Our findings suggest that the approximately 20% increase in biomass production reported in our previous study can be attributed to the synergistic effects of enhanced L/D cycle frequencies and improved light availability resulting from the transfer of cells from dark to light-limited zones. To further enhance the effectiveness of VIV, design improvements were developed. 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Improving light availability and creating high-frequency light-dark cycles in raceway ponds through vortex-induced vibrations for microalgae cultivation: a fluid dynamic study.
Limited light availability due to insufficient vertical mixing strongly reduces the applicability of raceway ponds (RWPs). To overcome this and create light-dark (L/D) cycles for enhanced biomass production through improved vertical mixing, vortex-induced vibration (VIV) system was implemented by the authors in a previous study to an existing pilot-scale RWP. In this study, experimental characterization of fluid dynamics for VIV-implemented RWP is carried out. Particle image velocimetry (PIV) technique is applied to visualize the flow. The extents of the vertical mixing due to VIV and the characteristics of L/D cycles were examined by tracking selected particles. Pond depth was hypothetically divided into three zones, namely dark, light Iimited and light saturated for detailed analysis of cell trajectories. It has been observed that VIV cylinder oscillation can efficiently facilitate the transfer of cells from light-limited to light-saturated zones. Among the cells that were tracked, 44% initially at dark zone entered the light-limited zone and 100% of initially at light-limited zone entered the light-saturated zone. 33% of all tracked cells experienced high-frequency L/D cycles with an average frequency of 35.69 s-1 and 0.49 light fraction. The impact of VIV was not discernible in the deeper sections of the pond, due to constrained oscillation amplitudes. Our findings suggest that the approximately 20% increase in biomass production reported in our previous study can be attributed to the synergistic effects of enhanced L/D cycle frequencies and improved light availability resulting from the transfer of cells from dark to light-limited zones. To further enhance the effectiveness of VIV, design improvements were developed. It was concluded that light availability could be significantly improved with the presented method for more effective use of RWPs.
期刊介绍：
Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes.
Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged.
The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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