基于统计实验设计,使用基于萜烯的深共晶溶剂从柠檬残渣中有效回收富含柠檬烯的浓缩物。

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Phytochemical Analysis Pub Date : 2024-12-01 Epub Date: 2024-08-01 DOI:10.1002/pca.3431
Melike Meryem Horuzoğlu, Sefanur Satılmış, Ebru Kurtulbaş, Selin Şahin
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引用次数: 0

摘要

导言:考虑到果皮占水果总重量的近一半,果汁工业的废弃副产品对于循环经济概念似乎很有价值。因此,从相关生物废料中回收这些高价值成分已成为一个非常有趣的研究课题:材料与方法:使用均质-异构萃取(HAE)从柠檬皮中回收富含柠檬烯(挥发性混合物的主要成分)的柑橘提取物。以薄荷醇为基础的深共晶混合物与羧酸(甲酸、乙酸和丙酸)一起使用。在以不同摩尔比(1/1、1/2 和 2/1)制备的溶剂中,效率最高(以柠檬烯含量计)的组合继续进行优化。通过响应面法(RSM)对工艺参数进行分析,以优化工艺。采用气相色谱-质谱(GC-MS)和固相微萃取(SPME)技术对 D-柠檬烯的产量进行了定量。柠檬皮提取物的质量还通过体外生物活性检测(酚含量和 2,2-二苯基-1-苦基肼 [DPPH] 自由基清除活性)进行了评估:在 2 毫克固体/30 毫升 DES、~53 秒和 ~8500 转/分的条件下,产量最高(每克新鲜样品中含 3.80 毫克柠檬烯)。从统计学角度看,最有效的变量是固体质量,其次是混合速度和萃取时间的次幂,p 值为 0.05。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effective recovery of limonene-rich concentrate from lemon residues using terpene-based deep eutectic solvents based on statistical experimental design.

Introduction: Waste by-products of the juice industry appear valuable for the circular economy concept, considering that the peel accounts for almost half of the total fruit weight. Therefore, the recovery of these highly valuable components from relevant biowaste has become a very interesting research topic.

Objective: The current study aims to develop an extraction process integrated with hydrophobic deep eutectic solvent (DES) based on statistical experimental design approach.

Material and methods: Homogenizer-assissted extraction (HAE) was used to recover the citrus extract rich in limonene (the main component of the volatile mixture) from lemon peels. Menthol-based deep eutectic mixtures were accompanied by carboxylic acids (formic, acetic, and propionic acids). Optimization continued on the combination that gave the highest efficiency (in terms of limonene content) among the solvents prepared at different molar ratios (1/1, 1/2, and 2/1). Process parameters were analyzed to optimize the process through central composite design with response surface method (RSM). D-Limonene yield was quantified with gas chromatography-mass spectrometry (GC-MS) with solid-phase microextraction (SPME) technique. The quality of the lemon peel extracts was also evaluated with respect to in vitro bioactivity assays (phenolic content and 2,2-diphenyl-1-picrylhydrazyl [DPPH] free radical scavenging activity).

Results: The maximum yield (3.80 mg-limonene per g fresh sample) was achieved by 2 mg solid/30 mL DES, ~53 sec, and ~8500 rpm. Statistically most effective variable was identified as solid mass, followed by second powers of mixing speed and extraction time at p < 0.0001.

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来源期刊
Phytochemical Analysis
Phytochemical Analysis 生物-分析化学
CiteScore
6.00
自引率
6.10%
发文量
88
审稿时长
1.7 months
期刊介绍: Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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