蛋白接枝弱凝胶能有效稳定高内相乳液并干扰脂质水解:作为疏水性活性物质的输送载体

Mingyue Yang, Nan Xiao, Shanguang Guo, Minmin Ai
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引用次数: 0

摘要

本研究考察了蛋白接枝(EWG)弱凝胶稳定负载姜黄素的高内相乳液(HIPE)的性能,并通过质子核磁共振 1H NMR 分析了消化后的脂质水解情况。结果表明,加热稳定 HIPE 后形成的 EWG 弱凝胶具有较小的粒径、较高的储存模量和损失模量值、紧凑的微观结构以及较好的环境稳定性。经 EWG 弱凝胶稳定化的 HIPE 中封装的姜黄素的稳定性提高了 1.80 倍。在体外模拟消化中,随着 EWG 弱凝胶比例的增加,HIPEs 释放的游离脂肪酸从 89.59% 降至 31.85%。根据 1H NMR 分析结果,不饱和脂质在加热后形成的 EWG 弱凝胶所形成的较厚界面层的保护下,不易水解和氧化。因此,EWG 弱凝胶可以稳定 HIPE,有效输送疏水性生物活性化合物,并干扰脂质水解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Egg white grafts weak‐gels efficiently stabilize high internal phase emulsions and interfere with lipid hydrolysis: As delivery vehicles for hydrophobic actives
This study investigated the performance of egg white grafts (EWG) weak‐gel to stabilize high internal phase emulsions (HIPEs) loaded with curcumin and analyzed the lipids hydrolysis after digestion via proton nuclear magnetic resonance 1H NMR. The results showed that the EWG weak‐gel formed after heating stabilized HIPEs exhibited small particle sizes, high values of storage and loss modulus, and a compact microstructure as well as better environmental stability. The stability of curcumin encapsulated in HIPEs stabilized by EWG weak‐gel was increased by 1.80‐fold. In in vitro simulation of digestion, the release of free fatty acids by HIPEs decreased from 89.59% to 31.85% with the increasing EWG weak‐gel proportion. Based on the results of 1H NMR analysis, unsaturated lipids are less susceptible to hydrolysis and oxidation as they are protected by a thicker interfacial layer formed by the EWG weak‐gel formed after heating. Hence, the EWG weak‐gel can stabilize HIPEs and deliver hydrophobic bioactive compounds efficiently and interfere with lipids hydrolysis.
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