筑雪皂苷 IVa 对过氧化氢诱导的 IPEC-J2 细胞损伤的保护作用

IF 1.5 4区医学 Q4 CHEMISTRY, MEDICINAL

Natural Product Communications Pub Date : 2024-07-25 DOI:10.1177/1934578x241262895

Qing Liu, Yingfeng Ma, Hanxiao Fan, Jialu Li, Jinbao Tang, Tianjie Wang, Ying Fan, Jianming Su, Hongyu Lei

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引用次数: 0

摘要

研究目的筑雪皂苷Ⅳa（CHS-Ⅳa）是一种广泛存在于三七等中草药中的皂苷化合物，具有多种功能。本研究旨在探讨 CHS-IVa 在过氧化氢（H2O2）作用下对猪肠上皮细胞（IPEC-J2）氧化应激和炎症的保护作用。研究方法用 MTT 法检测细胞活力。细胞划痕实验用于检测 CHS-IVa 对细胞的修复能力。使用相关试剂盒检测乳酸脱氢酶（LDH）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、硫氧还原酶（TrxR）和丙二醛（MDA）的水平。通过 qPCR 检测 TNF-α、NF-κB、IL-6、IL-1α 和 P65 的表达水平。结果显示MTT 结果显示，50 至 100 μg/mL 的 CHS-IVa 可在 24 小时内增强 IPEC-J2 细胞的活性。80 和 100 μg/mL 的 CHS-IVa 能促进损伤修复，并有效抑制 IPEC-J2 细胞在暴露于 H2O2 后释放 LDH。此外，它还对 TrxR、SOD、GSH 和 CAT 的损失有修复作用。同时，CHS-IVA 还能显著降低 H2O2 处理后 NF-κB、IL-6 和 P65 的 mRNA 表达。结论这些研究结果表明，特定浓度的 CHS-IVa 在氧化应激过程中对 IPEC 有保护作用，从而增强其修复损伤的能力。

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Protective Effect of Chikusetsu saponin IVa on Hydrogen Peroxide Induced Injury of IPEC-J2 Cells

Objectives: Chikusetsu saponin IVa (CHS-IVa) is a saponin compound widely found in herbs such as Panax japonicus C.A.Mey, which has various functions. The purpose of this study was to investigate the protective effect of CHS-IVa on oxidative stress and inflammation in porcine intestinal epithelial cells (IPEC-J2) under the action of hydrogen peroxide (H2O2). Methods: Cell viability was detected by MTT method. The cell scratch experiment was used to test the repair ability of CHS-IVa on cells. The levels of lactate dehydrogenase (LDH), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), thioredoxin reductase (TrxR), and malondialdehyde (MDA) were tested using the relevant kits. The expression levels of TNF-α, NF-κB, IL-6, IL-1α, and P65 were detected by qPCR. Results: MTT results showed that CHS-IVa at 50 to 100 μg/mL could enhance the activity of IPEC-J2 cells within 24 hours. CHS-IVa at 80 and 100 μg/mL could promote damage repair and effectively inhibit the release of LDH production in IPEC-J2 cells after exposure to H2O2. Additionally, it had a restorative effect on the loss of TrxR, SOD, GSH, and CAT. At the same time, the treatment of CHS-IVa significantly decreased the mRNA expression of NF-κB, IL-6, and P65 after H2O2 treatment. Conclusion: These findings indicate that a specific concentration of CHS-IVa has a protective effect on IPEC during oxidative stress, thereby enhancing their ability to repair damage.

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来源期刊

Natural Product Communications 工程技术-食品科技

CiteScore

3.10

自引率

11.10%

发文量

254

审稿时长

2.7 months

期刊介绍： Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products.