基于一次性不锈钢电化学免疫传感器的番茄叶脱落酸体内检测技术

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2024-07-25 DOI:10.1021/acs.jafc.4c03594

Lingjuan Tang, Zhiyao Zhang, Ling Sun, Xu Gao, Xinyue Zhao, Xinru Chen, Xingyu Zhu, Aixue Li, Lijun Sun

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引用次数: 0

摘要

脱落酸（ABA）在植物体内发挥着重要的调节作用。原位获取 ABA 的动态变化对植物学研究至关重要。本文结合纸质分析装置，开发了基于一次性不锈钢片的电化学免疫电极，用于植物中 ABA 的原位检测。不锈钢片上添加了碳水泥、二茂铁-氧化石墨烯-多壁碳纳米管纳米复合材料和 ABA 抗体。该系统可检测 1 nM 至 100 μM 范围内的 ABA，检测限为 100 pM。该系统原位检测了高盐度条件下番茄叶片中的 ABA 含量。ABA 的变化趋势与 SlNCED1 和 SlNCED2 的表达相似。总之，本研究提供了一种原位检测植物体内 ABA 的方法，有助于研究植物体内 ABA 的调控机制，促进精准农业的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

In Vivo Detection of Abscisic Acid in Tomato Leaves Based on a Disposable Stainless Steel Electrochemical Immunosensor

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In Vivo Detection of Abscisic Acid in Tomato Leaves Based on a Disposable Stainless Steel Electrochemical Immunosensor

Abscisic acid (ABA) plays an important regulatory role in plants. It is very critical to obtain the dynamic changes of ABA in situ for botanical research. Herein, coupled with paper-based analysis devices, electrochemical immunoelectrodes based on disposable stainless steels sheet were developed for ABA detection in plants in situ. The stainless steel sheets were modified with carbon cement, ferrocene-graphene oxide-multi walled carbon nanotubes nanocomposites, and ABA antibodies. The system can detect the ABA in the range of 1 nM to 100 μM, with a limit of detection of 100 pM. The ABA content in tomato leaves under high salinity was detected in situ. The trend of ABA changes was similar to the expression of SlNCED1 and SlNCED2. Overall, this study offers an approach for in situ detection of ABA in plants, which will help to study the regulation mechanism of ABA in plants and to promote the development of precision agriculture.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.