Lucrezia Angeli, Ksenia Morozova, Corinna Dawid, Matteo Scampicchio and Timo D. Stark*,
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引用次数: 0
摘要
意大利藏红花(Crocus sativus L.)因其高品质和种植地区的差异而日益受到关注。在这项研究中,采用 UPLC-ESI-TOF MS 同时采集低碰撞能量和高碰撞能量质谱(MSe)的非靶向代谢组学方法,对从当地生产商和超市购买的意大利藏红花样品的代谢物组成和质量进行了研究。无监督统计方法(PCA)凸显了代谢组中的显著差异,即使这些差异与地理来源无关。OPLS-DA 发现,9(S)-,10-(S)-,13-(S)-三羟基-11-(E)-十八碳烯酸是区分超市藏红花的最重要化合物,而氧化鳄鱼毒素则是进一步描述藏红花质量的最有价值的标记,即使在当地生产的样品中也是如此。已知的辣椒粉和姜黄掺假检测限为 10%,环姜黄素信号的增加是姜黄污染的重要生物标记。传统的和动力学的抗氧化检测也证实了这一结果。
UPLC-ESI-TOF MS Profiling Discriminates Biomarkers in Authentic and Adulterated Italian Samples of Saffron (Crocus sativus L.)
Italian saffron (Crocus sativus L.) is gaining visibility due to its high quality and difference in growing area. In this study, the metabolite composition and quality of Italian saffron samples purchased from local producers and supermarkets were investigated using an untargeted metabolomics approach using UPLC-ESI-TOF MS with simultaneous acquisition of low- and high-collision energy mass spectrometry (MSe). Unsupervised statistical method (PCA) highlighted significant differences in the metabolomes, even if not related to the geographical origin. OPLS-DA revealed 9(S)-,10-(S)-,13-(S)-tri-hydroxy-11-(E)-octadecenoic acid as the most decisive compound to distinguish supermarket saffron, while oxidized crocins represented the most valuable markers to further describe the quality of saffron, even in locally produced samples. Known adulterations with paprika and turmeric were detected at a limit of 10%, and the increasing signals of cyclocurcumin was a significant biomarker for turmeric contamination. The results were underlined with conventional and kinetic antioxidant assays.