Mayara Silva Pedro, Santino Aleandro da Silva, Luiz Henrique Picoli, Lucas Santana da Cunha, Andressa Cristina Zamboni Machado
{"title":"评估大豆对 Pratylenchus brachyurus 反应的实验和统计方法","authors":"Mayara Silva Pedro, Santino Aleandro da Silva, Luiz Henrique Picoli, Lucas Santana da Cunha, Andressa Cristina Zamboni Machado","doi":"10.1007/s40858-024-00665-1","DOIUrl":null,"url":null,"abstract":"<p>In Brazilian soybean production, the main annual yield losses are caused by nematodes. One of the principal plant-parasitic nematodes is the root-lesion nematode, <i>Pratylenchus brachyurus</i>. Resistance is among the major tools to manage nematodes, but the correct characterization of genotypes is a challenge. Several factors contribute to accurate phenotyping for nematode resistance, including the density of inoculum, the age of the plant at inoculation, and the timing of plant evaluation. Therefore, the objective of this study was to suggest standardizing the initial inoculum population and plant age at the time of <i>P. brachyurus</i> inoculation. We evaluated the population dynamics of <i>P. brachyurus</i> in a susceptible soybean cultivar until the 100th day after the inoculation based on the nematode reproduction factor (RF) weekly assessment. RF values were low at 30 DAI, and only after 51 DAI, the reproduction of <i>P. brachyurus</i> was sufficient to ensure more precise RF values in the susceptible soybean evaluated. Soybean plants inoculated at different plant ages (0, 10, and 20 days after planting) and inoculum densities (200, 400, 800, 1,600, and 3,200 nematodes per plant) were assessed 70 days after inoculation (DAI) to RF. Based on our data, we recommend that for an accurate classification of soybean genotypes in relation to resistance or susceptibility to <i>P. brachyurus</i>, inoculation should be conducted with 200 to 800 nematodes per plant within 7 to 15 days after sowing, and the evaluation should be done at least 70 days after inoculation.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":"21 1","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Experimental and statistical approaches to evaluate the reaction of soybean to Pratylenchus brachyurus\",\"authors\":\"Mayara Silva Pedro, Santino Aleandro da Silva, Luiz Henrique Picoli, Lucas Santana da Cunha, Andressa Cristina Zamboni Machado\",\"doi\":\"10.1007/s40858-024-00665-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>In Brazilian soybean production, the main annual yield losses are caused by nematodes. One of the principal plant-parasitic nematodes is the root-lesion nematode, <i>Pratylenchus brachyurus</i>. Resistance is among the major tools to manage nematodes, but the correct characterization of genotypes is a challenge. Several factors contribute to accurate phenotyping for nematode resistance, including the density of inoculum, the age of the plant at inoculation, and the timing of plant evaluation. Therefore, the objective of this study was to suggest standardizing the initial inoculum population and plant age at the time of <i>P. brachyurus</i> inoculation. We evaluated the population dynamics of <i>P. brachyurus</i> in a susceptible soybean cultivar until the 100th day after the inoculation based on the nematode reproduction factor (RF) weekly assessment. RF values were low at 30 DAI, and only after 51 DAI, the reproduction of <i>P. brachyurus</i> was sufficient to ensure more precise RF values in the susceptible soybean evaluated. Soybean plants inoculated at different plant ages (0, 10, and 20 days after planting) and inoculum densities (200, 400, 800, 1,600, and 3,200 nematodes per plant) were assessed 70 days after inoculation (DAI) to RF. Based on our data, we recommend that for an accurate classification of soybean genotypes in relation to resistance or susceptibility to <i>P. brachyurus</i>, inoculation should be conducted with 200 to 800 nematodes per plant within 7 to 15 days after sowing, and the evaluation should be done at least 70 days after inoculation.</p>\",\"PeriodicalId\":23354,\"journal\":{\"name\":\"Tropical Plant Pathology\",\"volume\":\"21 1\",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical Plant Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s40858-024-00665-1\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s40858-024-00665-1","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
摘要
在巴西的大豆生产中,每年的主要产量损失都是由线虫造成的。主要的植物寄生线虫之一是根线虫(Pratylenchus brachyurus)。抗性是管理线虫的主要手段之一,但正确鉴定基因型是一项挑战。有几个因素会影响线虫抗性表型的准确性,包括接种密度、接种时的植株年龄以及植株评估的时间。因此,本研究的目的是建议对接种 P. brachyurus 时的初始接种体数量和植株年龄进行标准化。我们根据线虫繁殖系数(RF)的每周评估结果,评估了一种易感大豆栽培品种在接种后第 100 天前的 P. brachyurus 种群动态。在 30 DAI 时,RF 值较低,只有在 51 DAI 后,P. brachyurus 的繁殖才足以确保在所评估的易感大豆中获得更精确的 RF 值。在不同株龄(播种后 0、10 和 20 天)和接种密度(每株 200、400、800、1,600 和 3,200 条线虫)下接种的大豆植株在接种后 70 天(DAI)进行 RF 评估。根据我们的数据,我们建议,为了准确划分大豆基因型对 P. brachyurus 的抗性或易感性,应在播种后 7 至 15 天内接种每株 200 至 800 头线虫,并在接种后至少 70 天进行评估。
Experimental and statistical approaches to evaluate the reaction of soybean to Pratylenchus brachyurus
In Brazilian soybean production, the main annual yield losses are caused by nematodes. One of the principal plant-parasitic nematodes is the root-lesion nematode, Pratylenchus brachyurus. Resistance is among the major tools to manage nematodes, but the correct characterization of genotypes is a challenge. Several factors contribute to accurate phenotyping for nematode resistance, including the density of inoculum, the age of the plant at inoculation, and the timing of plant evaluation. Therefore, the objective of this study was to suggest standardizing the initial inoculum population and plant age at the time of P. brachyurus inoculation. We evaluated the population dynamics of P. brachyurus in a susceptible soybean cultivar until the 100th day after the inoculation based on the nematode reproduction factor (RF) weekly assessment. RF values were low at 30 DAI, and only after 51 DAI, the reproduction of P. brachyurus was sufficient to ensure more precise RF values in the susceptible soybean evaluated. Soybean plants inoculated at different plant ages (0, 10, and 20 days after planting) and inoculum densities (200, 400, 800, 1,600, and 3,200 nematodes per plant) were assessed 70 days after inoculation (DAI) to RF. Based on our data, we recommend that for an accurate classification of soybean genotypes in relation to resistance or susceptibility to P. brachyurus, inoculation should be conducted with 200 to 800 nematodes per plant within 7 to 15 days after sowing, and the evaluation should be done at least 70 days after inoculation.
期刊介绍:
Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.
Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection.
The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines.
Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.