墨西哥锡那罗亚州和索诺拉州鹰嘴豆枯萎病相关真菌的系统发育、分布和致病性

IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences
C. I. Cota-Barreras, R. S. García-Estrada, J. León-Félix, V. Valenzuela-Herrera, G. A. Mora-Romero, K. Y. Leyva-Madrigal, J. M. Tovar-Pedraza
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引用次数: 0

摘要

枯萎病复合病害是墨西哥产区鹰嘴豆(Cicer arietinum L.)最重要的病害。病害症状包括根部腐烂、枯黄、萎蔫、生长不良、维管束组织变色和植株死亡。本研究旨在通过系统发育分析和形态特征描述相结合的方法,确定与墨西哥西北部鹰嘴豆枯萎病相关的镰刀菌种,并确定其对鹰嘴豆幼苗的致病性和侵染性。从分布在墨西哥锡那罗亚州和索诺拉州的 16 块鹰嘴豆田中有症状的植株上共分离出 80 株真菌。随后,选取了代表地理来源范围的 41 个分离物子集进行进一步的形态特征、系统发育和致病性测试。通过对翻译延伸因子 1-α(tef1-α)和 RNA 聚合酶第二大亚基(rpb2)基因的部分片段进行系统发育分析,确定了 26 个镰刀菌分离物和 15 个新孢子菌分离物的物种级别。在温室条件下对鹰嘴豆幼苗(Blanco Sinaloa 栽培品种)进行了致病性试验。本研究对 41 个真菌分离物进行了系统发育分析,确定了 Fusarium languescens(51.2%)、Neocosmospora falciformis(36.6%)、F. nirenbergiae(7.3%)和 F. verticillioides(4.9%)。发现所有真菌分离物对鹰嘴豆幼苗都具有致病性,且侵染性差异显著。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Phylogeny, distribution, and pathogenicity of fusarioid fungi associated with chickpea wilt in Sinaloa and Sonora, Mexico

Phylogeny, distribution, and pathogenicity of fusarioid fungi associated with chickpea wilt in Sinaloa and Sonora, Mexico

Wilt disease complex is the most important disease of chickpeas (Cicer arietinum L.) in the production areas from Mexico. Disease symptoms include root rot, yellowing, wilting, poor growth, discoloration of vascular tissues, and death of plants. This study aimed to identify the fusarioid species associated with chickpea wilt in northwest Mexico by the combination of phylogenetic analyses and morphological characterization, as well as to determine their pathogenicity and aggressiveness on chickpea seedlings. A total of 80 isolates of fusarioid fungi were obtained from symptomatic plants of 16 chickpea fields distributed in Sinaloa and Sonora, Mexico. Subsequently, a subset of 41 isolates representing the range of geographic origin was selected for further morphological characterization, phylogeny, and pathogenicity tests. Phylogenetic analyses of partial fragments of the translation elongation factor 1-alpha (tef1-α) and RNA polymerase second largest subunit (rpb2) genes were used to determine the identity of 26 Fusarium isolates and 15 Neocosmospora isolates to species level. Pathogenicity tests were performed on chickpea seedlings (cv. Blanco Sinaloa) under greenhouse conditions. Phylogenetic analyses of 41 fungal isolates of this study allowed the identification of Fusarium languescens (51.2%), Neocosmospora falciformis (36.6%), F. nirenbergiae (7.3%), and F. verticillioides (4.9%). All fungal isolates were found to be pathogenic on chickpea seedlings and a significant difference in aggressiveness was observed.

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来源期刊
Tropical Plant Pathology
Tropical Plant Pathology PLANT SCIENCES-
CiteScore
4.50
自引率
4.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.  Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection. The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines. Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.
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