C. I. Cota-Barreras, R. S. García-Estrada, J. León-Félix, V. Valenzuela-Herrera, G. A. Mora-Romero, K. Y. Leyva-Madrigal, J. M. Tovar-Pedraza
{"title":"墨西哥锡那罗亚州和索诺拉州鹰嘴豆枯萎病相关真菌的系统发育、分布和致病性","authors":"C. I. Cota-Barreras, R. S. García-Estrada, J. León-Félix, V. Valenzuela-Herrera, G. A. Mora-Romero, K. Y. Leyva-Madrigal, J. M. Tovar-Pedraza","doi":"10.1007/s40858-024-00663-3","DOIUrl":null,"url":null,"abstract":"<p>Wilt disease complex is the most important disease of chickpeas (<i>Cicer arietinum</i> L.) in the production areas from Mexico. Disease symptoms include root rot, yellowing, wilting, poor growth, discoloration of vascular tissues, and death of plants. This study aimed to identify the fusarioid species associated with chickpea wilt in northwest Mexico by the combination of phylogenetic analyses and morphological characterization, as well as to determine their pathogenicity and aggressiveness on chickpea seedlings. A total of 80 isolates of fusarioid fungi were obtained from symptomatic plants of 16 chickpea fields distributed in Sinaloa and Sonora, Mexico. Subsequently, a subset of 41 isolates representing the range of geographic origin was selected for further morphological characterization, phylogeny, and pathogenicity tests. Phylogenetic analyses of partial fragments of the translation elongation factor 1-alpha (<i>tef1-α</i>) and RNA polymerase second largest subunit (<i>rpb2</i>) genes were used to determine the identity of 26 <i>Fusarium</i> isolates and 15 <i>Neocosmospora</i> isolates to species level. Pathogenicity tests were performed on chickpea seedlings (cv. Blanco Sinaloa) under greenhouse conditions. Phylogenetic analyses of 41 fungal isolates of this study allowed the identification of <i>Fusarium languescens</i> (51.2%)<i>, Neocosmospora falciformis</i> (36.6%), <i>F. nirenbergiae</i> (7.3%), and <i>F. verticillioides</i> (4.9%). All fungal isolates were found to be pathogenic on chickpea seedlings and a significant difference in aggressiveness was observed.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phylogeny, distribution, and pathogenicity of fusarioid fungi associated with chickpea wilt in Sinaloa and Sonora, Mexico\",\"authors\":\"C. I. Cota-Barreras, R. S. García-Estrada, J. León-Félix, V. Valenzuela-Herrera, G. A. Mora-Romero, K. Y. Leyva-Madrigal, J. M. Tovar-Pedraza\",\"doi\":\"10.1007/s40858-024-00663-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Wilt disease complex is the most important disease of chickpeas (<i>Cicer arietinum</i> L.) in the production areas from Mexico. Disease symptoms include root rot, yellowing, wilting, poor growth, discoloration of vascular tissues, and death of plants. This study aimed to identify the fusarioid species associated with chickpea wilt in northwest Mexico by the combination of phylogenetic analyses and morphological characterization, as well as to determine their pathogenicity and aggressiveness on chickpea seedlings. A total of 80 isolates of fusarioid fungi were obtained from symptomatic plants of 16 chickpea fields distributed in Sinaloa and Sonora, Mexico. Subsequently, a subset of 41 isolates representing the range of geographic origin was selected for further morphological characterization, phylogeny, and pathogenicity tests. Phylogenetic analyses of partial fragments of the translation elongation factor 1-alpha (<i>tef1-α</i>) and RNA polymerase second largest subunit (<i>rpb2</i>) genes were used to determine the identity of 26 <i>Fusarium</i> isolates and 15 <i>Neocosmospora</i> isolates to species level. Pathogenicity tests were performed on chickpea seedlings (cv. Blanco Sinaloa) under greenhouse conditions. Phylogenetic analyses of 41 fungal isolates of this study allowed the identification of <i>Fusarium languescens</i> (51.2%)<i>, Neocosmospora falciformis</i> (36.6%), <i>F. nirenbergiae</i> (7.3%), and <i>F. verticillioides</i> (4.9%). All fungal isolates were found to be pathogenic on chickpea seedlings and a significant difference in aggressiveness was observed.</p>\",\"PeriodicalId\":23354,\"journal\":{\"name\":\"Tropical Plant Pathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-06-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical Plant Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s40858-024-00663-3\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s40858-024-00663-3","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Phylogeny, distribution, and pathogenicity of fusarioid fungi associated with chickpea wilt in Sinaloa and Sonora, Mexico
Wilt disease complex is the most important disease of chickpeas (Cicer arietinum L.) in the production areas from Mexico. Disease symptoms include root rot, yellowing, wilting, poor growth, discoloration of vascular tissues, and death of plants. This study aimed to identify the fusarioid species associated with chickpea wilt in northwest Mexico by the combination of phylogenetic analyses and morphological characterization, as well as to determine their pathogenicity and aggressiveness on chickpea seedlings. A total of 80 isolates of fusarioid fungi were obtained from symptomatic plants of 16 chickpea fields distributed in Sinaloa and Sonora, Mexico. Subsequently, a subset of 41 isolates representing the range of geographic origin was selected for further morphological characterization, phylogeny, and pathogenicity tests. Phylogenetic analyses of partial fragments of the translation elongation factor 1-alpha (tef1-α) and RNA polymerase second largest subunit (rpb2) genes were used to determine the identity of 26 Fusarium isolates and 15 Neocosmospora isolates to species level. Pathogenicity tests were performed on chickpea seedlings (cv. Blanco Sinaloa) under greenhouse conditions. Phylogenetic analyses of 41 fungal isolates of this study allowed the identification of Fusarium languescens (51.2%), Neocosmospora falciformis (36.6%), F. nirenbergiae (7.3%), and F. verticillioides (4.9%). All fungal isolates were found to be pathogenic on chickpea seedlings and a significant difference in aggressiveness was observed.
期刊介绍:
Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.
Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection.
The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines.
Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.