{"title":"前列双歧杆菌调节乳酸以抑制大肠埃希氏菌扩增并缓解炎症性肠病","authors":"Xiaoxiang Gao , Yuxuan Xia , Xiaoxuan Sun , Wenxuan Dou , Ruimin Chen , Pan Huang , Leilei Yu , Fengwei Tian , Jianxin Zhao , Wei Chen , Qixiao Zhai","doi":"10.1016/j.fbio.2024.104626","DOIUrl":null,"url":null,"abstract":"<div><p>Inhibition of the abnormal proliferation of <em>Escherichia coli</em> is crucial for alleviating inflammatory bowel disease. Due to the complexity of the gut microbiota, specifically targeting and inhibiting particular microbes is a challenging task. One approach involves screening for probiotics that antagonize <em>E. coli</em>; however, this process often requires extensive <em>in vitro</em> experiments using a vast pool of probiotics. In this study, we employed a genome-scale metabolic prediction model to predict the interactions between 803 strains of microbes and <em>E. coli</em>. The results indicated that <em>Bifidobacterium breve</em> and <em>Bifidobacterium animalis</em> exhibited the most potent inhibitory effects. <em>In vitro</em> antibacterial assays and mouse experiments validated the inhibitory efficacy of <em>B. breve</em> CCFM1369. Additionally, <em>B. breve</em> CCFM1369 demonstrated the ability to inhibit key gene <em>Ldha</em> and <em>HIF-2α</em> and reduce the luminal lactate concentration, thereby diminishing the colonization capacity of <em>E. coli</em>. These findings support the potential application of <em>B. breve</em> in the alleviation of colitis.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bifidobacterium breve modulates lactic acid to curtail Escherichia coli expansion and alleviate inflammatory bowel disease\",\"authors\":\"Xiaoxiang Gao , Yuxuan Xia , Xiaoxuan Sun , Wenxuan Dou , Ruimin Chen , Pan Huang , Leilei Yu , Fengwei Tian , Jianxin Zhao , Wei Chen , Qixiao Zhai\",\"doi\":\"10.1016/j.fbio.2024.104626\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Inhibition of the abnormal proliferation of <em>Escherichia coli</em> is crucial for alleviating inflammatory bowel disease. Due to the complexity of the gut microbiota, specifically targeting and inhibiting particular microbes is a challenging task. One approach involves screening for probiotics that antagonize <em>E. coli</em>; however, this process often requires extensive <em>in vitro</em> experiments using a vast pool of probiotics. In this study, we employed a genome-scale metabolic prediction model to predict the interactions between 803 strains of microbes and <em>E. coli</em>. The results indicated that <em>Bifidobacterium breve</em> and <em>Bifidobacterium animalis</em> exhibited the most potent inhibitory effects. <em>In vitro</em> antibacterial assays and mouse experiments validated the inhibitory efficacy of <em>B. breve</em> CCFM1369. Additionally, <em>B. breve</em> CCFM1369 demonstrated the ability to inhibit key gene <em>Ldha</em> and <em>HIF-2α</em> and reduce the luminal lactate concentration, thereby diminishing the colonization capacity of <em>E. coli</em>. These findings support the potential application of <em>B. breve</em> in the alleviation of colitis.</p></div>\",\"PeriodicalId\":12409,\"journal\":{\"name\":\"Food Bioscience\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-06-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Bioscience\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212429224010563\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Bioscience","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212429224010563","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Bifidobacterium breve modulates lactic acid to curtail Escherichia coli expansion and alleviate inflammatory bowel disease
Inhibition of the abnormal proliferation of Escherichia coli is crucial for alleviating inflammatory bowel disease. Due to the complexity of the gut microbiota, specifically targeting and inhibiting particular microbes is a challenging task. One approach involves screening for probiotics that antagonize E. coli; however, this process often requires extensive in vitro experiments using a vast pool of probiotics. In this study, we employed a genome-scale metabolic prediction model to predict the interactions between 803 strains of microbes and E. coli. The results indicated that Bifidobacterium breve and Bifidobacterium animalis exhibited the most potent inhibitory effects. In vitro antibacterial assays and mouse experiments validated the inhibitory efficacy of B. breve CCFM1369. Additionally, B. breve CCFM1369 demonstrated the ability to inhibit key gene Ldha and HIF-2α and reduce the luminal lactate concentration, thereby diminishing the colonization capacity of E. coli. These findings support the potential application of B. breve in the alleviation of colitis.
Food BioscienceBiochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍:
Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.