PqqF 通过降低 Serratia marcescens FS14 的 pH 值来抑制 T6SS 的分泌。

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2024-01-09 DOI:10.1093/femsle/fnae047

Fengyu Jia, Xuede Peng, Xiaomei Yang, Shenshen Qiu, Shanshan Jia, Tingting Ran, Weiwu Wang, Dongqing Xu

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引用次数: 0

摘要

吡咯并喹啉醌（PQQ）是一种氧化还原辅助因子，具有许多重要的生理功能，Ⅵ型分泌系统（T6SS）普遍存在于革兰氏阴性菌中，在细菌的生理代谢中发挥着重要作用。本研究发现，在 M9 培养基中，pqqF 的缺失增强了 Serratia marcesens FS14 中 Hcp-1 的分泌。转录分析表明，缺失 pqqF 对 T6SS-1 的表达几乎没有影响。进一步研究发现，FS14 中葡萄糖脱氢酶催化的反应改变了培养基的 pH 值，从而增加了 Hcp-1 的分泌。最后，我们证明了培养基 pH 值的降低对 T6SS-1 分泌的抑制作用与 PQQ 诱导的抑制作用相似。在 FS14 中，pH 对 T6SS 的这种调控模式与之前在其他细菌中报道的不同。因此，我们的研究结果表明了 S. marcesens FS14 中 T6SS 的一种新的 pH 调节模式，并将拓宽我们对 T6SS 分泌调节的认识。

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PqqF inhibits T6SS secretion by decreasing the pH in Serratia marcescens FS14.

Pyrroloquinoline quinone (PQQ) is a redox cofactor with numerous important physiological functions, and the type VI secretion system (T6SS) is commonly found in Gram-negative bacteria and plays important roles in physiological metabolism of the bacteria. In this study, we found that the deletion of pqqF enhanced the secretion of Hcp-1 in Serratia marcesens FS14 in M9 medium. Transcriptional analysis showed that the deletion of pqqF almost had no effect on the expression of T6SS-1. Further study revealed that the increased secretion of Hcp-1 was altered by the pH changes of the culture medium through the reaction catalyzed by the glucose dehydrogenases in FS14. Finally, we demonstrated that decreased pH of culture medium has similar inhibition effects as PQQ induced on the secretion of T6SS-1. This regulation mode on T6SS by pH in FS14 is different from previously reported in other bacteria. Therefore, our results suggest a novel pH regulation mode of T6SS in S. marcesens FS14, and would broaden our knowledge on the regulation of T6SS secretion.

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.