基于 HPLC/ESI-QTOF-MS/MS 的 Taxus × media 六种组织的非靶向代谢组学鉴定。

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Phytochemical Analysis Pub Date : 2024-10-01 Epub Date: 2024-06-13 DOI:10.1002/pca.3403
Hangyu Bai, Dengwu Li
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引用次数: 0

摘要

简介:紫杉介质(Taxus × media Rehder)以紫杉醇含量高而闻名,是工业化生产紫杉醇的主要来源。除紫杉醇外,紫杉介质还含有多种代谢产物,包括类黄酮、生物碱和萜类化合物,已被证明具有抗氧化、抗菌、消炎和免疫调节作用。然而,这些化合物作为 T. media 的关键代谢物尚未得到深入研究:对六种介壳虫组织的非靶向代谢组学分析为介壳虫代谢物的开发和利用提供了新的见解:方法:采用高效液相色谱-四极杆飞行时间质谱(HPLC-Q-TOF-MS/MS)和化学计量学技术对 T. media 六种组织的提取物进行分析:结果:利用可靠的 HPLC-Q-TOF-MS/MS 方法,我们在六种介壳虫组织中鉴定出 312 种化合物,其中包括 214 种以前未在介壳虫中报道过的化合物。为了确定不同组织中的特征化合物,我们进一步筛选了 34 种代谢物。KEGG 代谢途径分析表明,这些化合物主要出现在萜烯糖苷、黄烷和 O-甲基化类黄酮的代谢途径中:本研究利用基于 HPLC-QTOF-MS/MS 的代谢组学方法初步评估了介壳虫不同组织中的代谢物,为介壳虫的利用和管理提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
HPLC/ESI-QTOF-MS/MS based untargeted metabolomics authentication of Taxus × media six tissues.

Introduction: Taxus media (Taxus × media Rehder) is renowned for its high paclitaxel content, serving as a major source for industrial paclitaxel production. In addition to paclitaxel, T. media contains a diverse range of metabolites, including flavonoids, alkaloids, and terpenoids, which have been shown to possess antioxidant, antibacterial, anti-inflammatory, and immunomodulatory effects. However, these compounds have not been thoroughly studied as key metabolites in T. media.

Objective: The untargeted metabolomics analysis of six T. media tissues provides new insights into the development and utilization of T. media metabolites.

Method: The extracts from six tissues of T. media were analyzed and subjected to analysis using high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS/MS) and chemometric techniques.

Results: Using a reliable HPLC-Q-TOF-MS/MS method, we identified 312 compounds in six T. media tissues, including 214 previously unreported in T. media. To identify characteristic compounds across different tissues, 34 metabolites were further screened. KEGG metabolic pathway analysis revealed that these compounds primarily occur in the metabolic pathways of terpene glycosides, flavans, and O-methylated flavonoids.

Conclusion: This study initially utilized an HPLC-QTOF-MS/MS-based metabolomics approach to assess the metabolites in different tissues of T. media, providing a basis for their utilization and management.

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来源期刊
Phytochemical Analysis
Phytochemical Analysis 生物-分析化学
CiteScore
6.00
自引率
6.10%
发文量
88
审稿时长
1.7 months
期刊介绍: Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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