使用短波红外持续发光的硅基和砷化镓基成像设备的深层组织近红外-II 生物成像性能

IF 5.1 2区 材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY
Yafei Chen, Simona Spinelli and Zhengwei Pan
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引用次数: 0

摘要

在第二个近红外(NIR-II;950-1700 nm)窗口进行生物成像时,采用基于砷化镓的相机,在观察浅层结构(3 mm)时可显示出卓越的清晰度和分辨率,但在有效成像深层组织特征方面却存在不足。深部组织近红外-II成像性能不佳的主要原因是成像造影剂的缺陷,主要涉及其发光亮度和波长,而很少考虑砷化镓相机的缺陷。在这里,我们使用 MgGeO3:Yb3+ 短波红外(SWIR)持久发光荧光粉作为对比剂,在相同的成像条件下评估了 InGaAs 相机和硅 CCD 相机在厚鸡胸组织(5-20 毫米)、厚小鼠身体(10-20 毫米)和小鼠内部器官(胃肠道、肺部和肝脏)中的深部组织生物成像能力。尽管在检测 950-1100 纳米的 SWIR 光时,InGaAs 摄像机的量子效率(80%-85%)明显高于 Si 摄像机(5%-30%),但由于其固有的高暗电流的明显干扰,前者在成像深部组织特征时的整体性能明显较差,特别是在成像信号微弱的情况下。不过,当提供足够强的 SWIR 成像信号时,InGaAs 摄像机的清晰度要优于硅摄像机,即使在厚度为 10 毫米的鸡组织和小鼠胃中也是如此。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Deep-tissue NIR-II bioimaging performance of Si-based and InGaAs-based imaging devices using short-wave infrared persistent luminescence†

Deep-tissue NIR-II bioimaging performance of Si-based and InGaAs-based imaging devices using short-wave infrared persistent luminescence†

Deep-tissue NIR-II bioimaging performance of Si-based and InGaAs-based imaging devices using short-wave infrared persistent luminescence†

Bioimaging in the second near-infrared (NIR-II; 950–1700 nm) window, employing InGaAs-based cameras, exhibits superior clarity and resolution in visualizing shallow structures (<3 mm), yet it falls short in effectively imaging deep-tissue features. The subpar performance of deep-tissue NIR-II imaging is largely attributed to shortcomings in imaging contrast agents, primarily concerning their luminescence brightness and wavelengths, with minimal consideration given to deficiencies in InGaAs cameras. Here, we use a MgGeO3:Yb3+ short-wave infrared (SWIR) persistent luminescent phosphor emitting at 950–1100 nm as a contrast agent to assess the deep-tissue bioimaging capabilities of both an InGaAs camera and a Si CCD camera under identical imaging conditions in thick chicken breast tissues (5–20 mm), thick mice bodies (10–20 mm), and internal mice organs (gastrointestinal tracts, lungs, and livers). Despite the significantly higher quantum efficiency of the InGaAs camera (∼80–85%) compared to the Si camera (∼5–30%) in detecting 950–1100 nm SWIR light, the former exhibits notably inferior performance overall in imaging deep-tissue features, particularly in scenarios with faint imaging signals, attributable to the pronounced interference of its inherently high dark current. Nonetheless, when provided with sufficiently intense SWIR imaging signals, the InGaAs camera outperforms the Si camera in terms of clarity, even in chicken tissues of 10 mm thickness and in the stomachs of mice.

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来源期刊
Journal of Materials Chemistry C
Journal of Materials Chemistry C MATERIALS SCIENCE, MULTIDISCIPLINARY-PHYSICS, APPLIED
CiteScore
10.80
自引率
6.20%
发文量
1468
期刊介绍: The Journal of Materials Chemistry is divided into three distinct sections, A, B, and C, each catering to specific applications of the materials under study: Journal of Materials Chemistry A focuses primarily on materials intended for applications in energy and sustainability. Journal of Materials Chemistry B specializes in materials designed for applications in biology and medicine. Journal of Materials Chemistry C is dedicated to materials suitable for applications in optical, magnetic, and electronic devices. Example topic areas within the scope of Journal of Materials Chemistry C are listed below. This list is neither exhaustive nor exclusive. Bioelectronics Conductors Detectors Dielectrics Displays Ferroelectrics Lasers LEDs Lighting Liquid crystals Memory Metamaterials Multiferroics Photonics Photovoltaics Semiconductors Sensors Single molecule conductors Spintronics Superconductors Thermoelectrics Topological insulators Transistors
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