Mitra Shahnavazi, Vahid Azarpeykan, Alireza Einali
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引用次数: 0
摘要
研究了湿冷(5 ºC)和暖分层(25 ºC)期间蛋白质修饰和氨基酸代谢在开心果(Pistacia vera L.)果核打破休眠中的作用。冷分层果核的发芽率高达 97%,而热分层果核的发芽率较低(40%)。在冷处理期间,子叶和胚轴中的蛋白质在中性 pH 值下的溶解度增加,并伴随着蛋白质羰基化,而在温暖培养条件下,这些值会降低。冷分层和暖分层果仁的两种组织中都出现了氨基酸积累。精氨酸酶活性在低温分层果仁的两种组织中都有所增加,但在温暖处理过程中显著下降。在开心果果仁冷、暖分层过程中,两个器官的精氨酸脱羧酶活性都有所提高,但鸟氨酸氨基转移酶活性在这两个时期都有所下降。这些结果表明,在低温分层过程中,蛋白质溶解度的增加及其羰基化可能会诱导蛋白质动员和氨基酸积累,使其随后进入适当的代谢途径。因此,可以认为精氨酸酶对蛋白质的修饰和精氨酸的代谢是果仁在低温分层过程中发芽的特异性事件。
Protein carbonylation and arginine utilization in cold- and warm-stratified pistachio (Pistacia vera L.) kernels
The role of protein modifications and amino acid metabolism in the dormancy breaking of pistachio (Pistacia vera L.) kernels during moist chilling (5 ºC) and warm stratification (25 ºC) were studied. Cold-stratified kernels showed germination up to 97%, while warm-stratified ones had low germination (40%). Increased protein solubility at neutral pH was accompanied by protein carbonylation in both cotyledons and embryonic axes during cold treatment, whereas these values decreased under warm incubation. Amino acid accumulation occurred in both tissues of cold- and warm-stratified kernels. Arginase activity increased in both tissues of cold-stratified kernels but significantly declined during warm treatment. While arginine decarboxylase activity of both organs increased under cold and warm stratification of pistachio kernels, ornithine aminotransferase activity declined during these periods. These results show that increased protein solubility and its carbonylation during cold stratification may induce the protein mobilization and accumulation of amino acids for their subsequent direction to the proper metabolic pathways. In this way, protein modification and arginine metabolism by arginase can be considered germination-specific events during cold stratification of kernels.
期刊介绍:
Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry.
The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.