结合UPLC-QTOF-MS/MS和UPLC-DAD评价硫磺熏蒸白芍对三种四物汤配方总体质量的影响

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Phytochemical Analysis Pub Date : 2024-08-01 Epub Date: 2024-05-13 DOI:10.1002/pca.3379
Jun-Jie Wang, Cai-Feng Hao, Pei-Yao Huang, Xiang-Ling Qin, Shan-Shan Zhou, Jin-Di Xu, Qian Mao, Song-Lin Li, Ming Kong
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引用次数: 0

摘要

简介:用硫磺熏蒸白芍(PRA)可诱导其生物活性成分芍药苷向含硫衍生物亚硫酸芍药苷的化学转化,从而改变PRA的质量、生物活性、药代动力学和毒性。然而,硫磺熏蒸 PRA(S-PRA)如何影响含 PRA 复方制剂的质量尚未得到深入评估:我们打算评估 S-PRA 对三种四物汤(SWT)制剂,即煎剂(SWT-D)、颗粒剂(SWT-G)和混合物(SWT-M)总体质量的影响:采用UPLC-DAD多组分定量法比较了含S-PRA和含NS-PRA的SWT制剂中亚硫酸芍药苷和其他10种生物活性成分的转移率。应用基于UPLC-QTOF-MS/MS的目标代谢组学方法探讨了含S-PRA的SWT制剂中不同的含硫衍生物:结果:三种含S-PRA的SWT制剂中亚硫酸芍药苷的转移率均高于100%。此外,S-PRA 还提高了 5-羟甲基糠醛、1,2,3,4,6-O-五聚戊酰基葡萄糖在三种 SWT 配方中的转移率,而降低了芍药苷、白花前胡苷和阿魏酸的转移率。最初在 S-PRA 中发现的六种蒎烷单萜烯葡萄糖苷亚硫酸盐在三种含有 S-PRA 的 SWT 制剂中也能检测到。此外,在这三种含有 S-PRA 的配方中还新发现了七种酚酸亚硫酸盐,包括 (3Z)-6-亚硫酸-女贞苷、(3E)-6-亚硫酸-女贞苷、6,8-二亚硫酸-女贞苷、亚阿魏酸、新绿原酸亚硫酸盐、绿原酸亚硫酸盐和当归亚硫酸盐(或异构体):结论:在制备三种 SWT 制剂的过程中,S-PRA 会对亚硫酸芍药苷和其他生物活性成分的转移率产生不同程度的影响,进而影响制剂的整体质量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Integrating UPLC-QTOF-MS/MS and UPLC-DAD to evaluate the influence of sulfur-fumigated Paeoniae Radix Alba on the overall quality of three Si-Wu-Tang formulations.

Introduction: Sulfur-fumigation of Paeoniae Radix Alba (PRA) could induce the chemical transformation of its bioactive component paeoniflorin into a sulfur-containing derivative paeoniflorin sulfite, and thus alter the quality, bioactivities, pharmacokinetics, and toxicities of PRA. However, how sulfur-fumigated PRA (S-PRA) affects the quality of PRA-containing complex preparations has not been intensively evaluated.

Objectives: We intend to evaluate the influence of S-PRA on the overall quality of three kinds of Si-Wu-Tang (SWT) formulations, i.e., decoction (SWT-D), granule (SWT-G), and mixture (SWT-M).

Material and methods: An UPLC-DAD multi-components quantification method was used to compare the transfer rates of paeoniflorin sulfite and other 10 bioactive components between S-PRA-containing and NS-PRA-containing SWT formulations. An UPLC-QTOF-MS/MS-based target metabolomics approach was applied to explore the differential sulfur-containing derivatives in S-PRA-containing SWT formulations.

Results: The transfer rates of paeoniflorin sulfite in three S-PRA-containing SWT formulations were all higher than 100%. Moreover, S-PRA also increased the transfer rate of 5-hydroxymethylfurfural, 1,2,3,4,6-O-pentagalloylglucose, whereas decreased that of paeoniflorin, albiflorin, and ferulic acid in three SWT formulations. Six pinane monoterpene glucoside sulfites originally identified in S-PRA, were also detectable in three S-PRA-containing SWT formulations. In addition, seven phenolic acid sulfites including (3Z)-6-sulfite-ligustilide, (3E)-6-sulfite-ligustilide, 6,8-disulfite-ligustilide, ferulic acid sulfite, neochlorogenic acid sulfite, chlorogenic acid sulfite, and angelicide sulfite (or isomer) were newly identified in these three S-PRA-containing formulations.

Conclusion: S-PRA could differentially affect the transfer rate of paeoniflorin sulfite and other bioactive components during the preparation of three SWT formulations and subsequently the overall quality thereof.

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来源期刊
Phytochemical Analysis
Phytochemical Analysis 生物-分析化学
CiteScore
6.00
自引率
6.10%
发文量
88
审稿时长
1.7 months
期刊介绍: Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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