利用壳聚糖/海藻酸盐修饰的长春花提取物绿色合成银纳米粒子：对病原菌的杀菌活性和正常细胞系的细胞毒性分析

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY

Current pharmaceutical design Pub Date : 2024-04-25 DOI:10.2174/0113816128304460240408085736

Mahnaz Mohtasham, Alieh Rezagholizade-shirvan, Zahra Hojat Bonab, Mohammad Reza Amiryousefi, Majid Darroudi, Mobina Sadat Ahmadi Solimani, Sajad Yaghoobi, Samaneh Dolatabadi, Ahmad Ghasemi, Amir abbas Momtazi-Borojeni

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引用次数: 0

摘要

目的：本研究旨在确定改性银纳米粒子（AgNPs）的体外药理作用。背景：AgNPs 被认为是一种抗菌剂：AgNPs被认为是抗菌剂。然而，化学合成 AgNPs（cAgNPs）的细胞毒性提出了限制其使用的挑战：研究利用壳聚糖/海藻酸盐（Ch/ALG-gAgNPs）修饰的长春花提取物合成的 AgNPs 的抗菌效果和细胞毒性。研究目的本研究的目的是考察利用壳聚糖/金刚砂 AgNPS（Ch/ALG-gAgNPs）修饰的鸡冠花提取物合成的 AgNPs 的抗菌和细胞毒性效果：使用 TEM、DLS、XRD 和 FTIR 对纳米粒子进行表征。采用盘扩散法和微稀释法对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）耐药菌株进行 Ch/ALG-gAgNPs 抗菌分析。采用 MTT 法和附件素/PI 染色法分别测定了 NPs 对 L929 正常细胞活力和凋亡的影响。方法：使用 TEM、DLS、XRD 和 FTIR 对纳米颗粒进行表征。采用盘扩散法和微量稀释法对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）耐药菌株进行 Ch/ALG-gAgNPs 抗菌分析。采用 MTT 法和附件素/PI 染色法分别测定了 NPs 对 L929 正常细胞活力和凋亡的影响。结果理化表征结果表明，Ch/ALG-gAgNPs 为球形，分散均匀，大小在 50 至 500 nm 之间。Ch/ALG-gAgNPs 对微生物菌株生长的抑制作用呈剂量依赖性。Ch/ALG-gAgNPs 的抗菌效果明显高于 cAgNPs。当 Ch/ALG-gAgNPs 的浓度低于 50 μg/ml 时，对正常细胞的细胞毒性很小。Ch/ALG-gAgNPs 的细胞毒性效应低于 cAgNPs。流式细胞术和实时聚合酶链式反应结果表明，与使用 cAgNPs 处理细胞相比，有 Ch/ALG-gAgNPs 存在时细胞凋亡的百分比和 BAX 标记都有所下降。结论目前的研究结果介绍了可用于医药的新型壳聚糖/精氨酸修饰 gAgNPs。

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Green Synthesis of Silver Nanoparticles Using Cirsium Congestum Extract Modified by Chitosan/Alginate: Bactericidal Activity against Pathogenic Bacteria and Cytotoxicity Analysis in Normal Cell Line

Aim: The study aimed to determine in vitro pharmacological effects of modified Ag nanoparticles (AgNPs). Background: AgNPs are considered antimicrobial agents. However, the cytotoxicity of chemically synthesized AgNPs (cAgNPs) has raised challenges that limit their use. objective: To examine the antimicrobial and cytotoxicity effects of AgNPs synthesized using Cirsium Congestum extract modified by chitosan/alginate (Ch/ALG-gAgNPs). Objective: The purpose of the study was to examine the antimicrobial and cytotoxicity effects of AgNPs synthesized using Cirsium congestum extract modified by chitosan/alginate AgNPS (Ch/ALG-gAgNPs). method: Nanoparticles were characterized using TEM, DLS, XRD, and FTIR. Resistant strains of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used for the antimicrobial analysis of Ch/ALG-gAgNPs using disk diffusion and microdilution methods. The effects of NPs on cell viability and apoptosis in L929 normal cells were determined using MTT assay and annexin/PI staining, respectively. Methods: Nanoparticles were characterized using TEM, DLS, XRD, and FTIR. Resistant strains of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used for the antimicrobial analysis of Ch/ALG-gAgNPs using disc diffusion and microdilution methods. The effects of NPs on cell viability and apoptosis in L929 normal cells were determined using MTT assay and annexin/PI staining, respectively. Results: Physicochemical characterizations confirmed Ch/ALG-gAgNPs to be spherical and uniformly dispersed, and their size ranged from 50 to 500 nm. Ch/ALG-gAgNPs inhibited the growth of microbial strains in a dose-dependent manner. The antibacterial effect of Ch/ALG-gAgNPs was significantly higher than cAgNPs. The Ch/ALG-gAgNPs showed little cytotoxicity against normal cells at concentrations less than 50 μg/ml. Cytotoxicity effects of Ch/ALG-gAgNP were less than cAgNPs. Flow cytometry and real-time PCR results showed a decrease in apoptosis percentage and BAX marker in the presence of Ch/ALG-gAgNPs relative to when the cell was treated with cAgNPs. Conclusion: Current findings introduce novel gAgNPs modified with chitosan/alginate for use in medicine.

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来源期刊

Current pharmaceutical design 医学-药学

CiteScore

6.30

自引率

0.00%

发文量

302

审稿时长

2 months

期刊介绍： Current Pharmaceutical Design publishes timely in-depth reviews and research articles from leading pharmaceutical researchers in the field, covering all aspects of current research in rational drug design. Each issue is devoted to a single major therapeutic area guest edited by an acknowledged authority in the field. Each thematic issue of Current Pharmaceutical Design covers all subject areas of major importance to modern drug design including: medicinal chemistry, pharmacology, drug targets and disease mechanism.