{"title":"利用普通荞麦(Fagopyrum esculentum Moench)中的新型等位基因 GlbNB2 消除致敏种子蛋白 13S 球蛋白中的零重复亚基","authors":"Takeyuki Okada, Kohtaro Kimura, Naruha Goto, Tomoyuki Katsube-Tanaka","doi":"10.1016/j.fochms.2024.100205","DOIUrl":null,"url":null,"abstract":"<div><p>Common buckwheat (<em>Fagopyrum esculentum</em> Moench) seeds contain 13S globulin, the zero-repeat subunit of which is trypsin-resistant and allergenic. Here, its two novel alleles were analyzed for development of hypoallergenic plants. The <em>GlbNC</em> allele has a Miniature Inverted-repeat Transposable Element (MITE)-like insertion in the 4th exon. However, most of the insertion was spliced-out, resulting in accumulation of zero-repeat subunit in <em>GlbNC</em> homozygotes. Meanwhile, the <em>GlbNB2</em> has a 164-bp insertion in the 3rd exon, resulting in no accumulation of zero-repeat subunit in <em>GlbNB2</em> homozygotes (NB2_homo). Both the insertion sequences were predicted to form a hairpin-like structure, and that of <em>GlbNB2</em> was more rigid than that of <em>GlbNC</em>. Trypsin digestion in NB2_homo showed that the α polypeptide of Met-rich subunit is also hard to digest, that is a next target to eliminate for hypoallergenic buckwheat development.</p></div>","PeriodicalId":34477,"journal":{"name":"Food Chemistry Molecular Sciences","volume":"8 ","pages":"Article 100205"},"PeriodicalIF":4.1000,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666566224000121/pdfft?md5=1ec92eae80cad5ebbf8879b82264d3a0&pid=1-s2.0-S2666566224000121-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Elimination of zero-repeat subunit in allergenic seed protein 13S globulin using the novel allele GlbNB2 in common buckwheat (Fagopyrum esculentum Moench)\",\"authors\":\"Takeyuki Okada, Kohtaro Kimura, Naruha Goto, Tomoyuki Katsube-Tanaka\",\"doi\":\"10.1016/j.fochms.2024.100205\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Common buckwheat (<em>Fagopyrum esculentum</em> Moench) seeds contain 13S globulin, the zero-repeat subunit of which is trypsin-resistant and allergenic. Here, its two novel alleles were analyzed for development of hypoallergenic plants. The <em>GlbNC</em> allele has a Miniature Inverted-repeat Transposable Element (MITE)-like insertion in the 4th exon. However, most of the insertion was spliced-out, resulting in accumulation of zero-repeat subunit in <em>GlbNC</em> homozygotes. Meanwhile, the <em>GlbNB2</em> has a 164-bp insertion in the 3rd exon, resulting in no accumulation of zero-repeat subunit in <em>GlbNB2</em> homozygotes (NB2_homo). Both the insertion sequences were predicted to form a hairpin-like structure, and that of <em>GlbNB2</em> was more rigid than that of <em>GlbNC</em>. Trypsin digestion in NB2_homo showed that the α polypeptide of Met-rich subunit is also hard to digest, that is a next target to eliminate for hypoallergenic buckwheat development.</p></div>\",\"PeriodicalId\":34477,\"journal\":{\"name\":\"Food Chemistry Molecular Sciences\",\"volume\":\"8 \",\"pages\":\"Article 100205\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-04-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2666566224000121/pdfft?md5=1ec92eae80cad5ebbf8879b82264d3a0&pid=1-s2.0-S2666566224000121-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Chemistry Molecular Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666566224000121\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry Molecular Sciences","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666566224000121","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Elimination of zero-repeat subunit in allergenic seed protein 13S globulin using the novel allele GlbNB2 in common buckwheat (Fagopyrum esculentum Moench)
Common buckwheat (Fagopyrum esculentum Moench) seeds contain 13S globulin, the zero-repeat subunit of which is trypsin-resistant and allergenic. Here, its two novel alleles were analyzed for development of hypoallergenic plants. The GlbNC allele has a Miniature Inverted-repeat Transposable Element (MITE)-like insertion in the 4th exon. However, most of the insertion was spliced-out, resulting in accumulation of zero-repeat subunit in GlbNC homozygotes. Meanwhile, the GlbNB2 has a 164-bp insertion in the 3rd exon, resulting in no accumulation of zero-repeat subunit in GlbNB2 homozygotes (NB2_homo). Both the insertion sequences were predicted to form a hairpin-like structure, and that of GlbNB2 was more rigid than that of GlbNC. Trypsin digestion in NB2_homo showed that the α polypeptide of Met-rich subunit is also hard to digest, that is a next target to eliminate for hypoallergenic buckwheat development.