厄瓜多尔商用烟草植物上黄瓜花叶病毒的特征和基因组分析

IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences
Enny Ganchozo-Mendoza, Ayane F. Ferreira Quadros, Francisco Murilo Zerbini, Francisco J. Flores, Felipe R. Garcés-Fiallos
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引用次数: 0

摘要

烟草(Nicotiana tabacum L.)是茄科植物,是南美洲的特有物种,在世界各地均有种植。虽然烟草在厄瓜多尔不是一种常见的作物,但它对国家经济非常重要,也是重要的就业来源。病毒感染对烟草的破坏性特别大,因为它会降低烟草的产量和质量。2021 年 7 月,在厄瓜多尔瓜亚斯省和洛斯里奥斯省的四块商业烟田观察到了萎黄病、马赛克和叶片变形的症状。本研究旨在全面描述从出现症状的植株中获得的病毒分离物的特征。利用高通量测序(HTS)、不同开放阅读框的系统进化分析和有症状植株的透射电子显微镜(TEM),我们首次报告了从厄瓜多尔商业烟草植株中分离出的黄瓜花叶病毒(CMV)的分子特征和基因组序列。在烟草商业种植田中检测和识别 CMV 的能力有助于制定有效的控制策略,从而减轻对优质烟叶生产的限制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization and genome analysis of Cucumber mosaic virus on commercial tobacco plants in Ecuador

Tobacco (Nicotiana tabacum L.), an endemic species of South America that is grown worldwide, is a member of the Solanaceae family. Although it is not a common crop in Ecuador, tobacco is important to the nation's economy and a significant source of employment. Viral infections are particularly devastating to tobacco as they can reduce both its yield and quality. In July 2021, symptoms of chlorosis, mosaic and leaf distortion were observed in four commercial fields in the provinces of Guayas and Los Ríos in Ecuador. This study aims to comprehensively characterize a viral isolate obtained from symptomatic plants. Using high throughput sequencing (HTS), phylogenetic analysis of the different open reading frames, and transmission electron microscopy (TEM) of symptomatic plants, we report for the first time the molecular characterization and genome sequence of Cucumber mosaic virus (CMV) isolated from commercial tobacco plants in Ecuador. The ability to detect and identify CMV in tobacco commercial plant fields enables the development of efficient control strategies, thereby mitigating constraints on the production of high-quality tobacco leaves.

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来源期刊
Tropical Plant Pathology
Tropical Plant Pathology PLANT SCIENCES-
CiteScore
4.50
自引率
4.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.  Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection. The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines. Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.
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