与睡眠压力相关的大脑新皮层神经元亚型特异性转录组变化

IF 2.4 4区 医学 Q3 NEUROSCIENCES
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引用次数: 0

摘要

睡眠受睡眠压力的调节,睡眠压力在清醒时增加,在睡眠时消散。最近的研究表明,大脑新皮层是一个由不同层和投射特异性神经元亚型组成的六层结构,它通过转录调控参与睡眠压力的表征。在这里,我们利用单核 RNA 测序数据集研究了睡眠压力增加时新皮层神经元亚型的转录组变化,并预测了参与转录组变化的潜在细胞内和细胞间分子。我们发现,在新皮层谷氨酸能神经元亚型中,睡眠剥夺(SD)对第2层和第3层脑内神经元(L2/3 IT)转录组的影响最大。众所周知,突变体 SIK3(SLP)会增加睡眠压力,它的表达也会诱导 L2/3 IT 神经元转录组发生深刻变化。我们发现 Junb 是参与 SD 和 SIK3 (SLP) 表达改变 L2/3 IT 神经元转录组的候选转录因子。最后,我们推断了可能参与 SD 诱导的 L2/3 IT 神经元转录组改变的推定细胞间配体,包括 BDNF、LSAMP 和 PRNP。我们认为,在新皮层谷氨酸能神经元亚型中,L2/3 IT神经元的转录组受睡眠压力增加的影响最大,并确定了参与这种转录改变的假定分子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Neuronal subtype-specific transcriptomic changes in the cerebral neocortex associated with sleep pressure
Sleep is homeostatically regulated by sleep pressure, which increases during wakefulness and dissipates during sleep. Recent studies have suggested that the cerebral neocortex, a six-layered structure composed of various layer- and projection-specific neuronal subtypes, is involved in the representation of sleep pressure governed by transcriptional regulation. Here, we examined the transcriptomic changes in neuronal subtypes in the neocortex upon increased sleep pressure using single-nucleus RNA sequencing datasets and predicted the putative intracellular and intercellular molecules involved in transcriptome alterations. We revealed that sleep deprivation (SD) had the greatest effect on the transcriptome of layer 2 and 3 intratelencephalic (L2/3 IT) neurons among the neocortical glutamatergic neuronal subtypes. The expression of mutant SIK3 (SLP), which is known to increase sleep pressure, also induced profound changes in the transcriptome of L2/3 IT neurons. We identified Junb as a candidate transcription factor involved in the alteration of the L2/3 IT neuronal transcriptome by SD and SIK3 (SLP) expression. Finally, we inferred putative intercellular ligands, including BDNF, LSAMP, and PRNP, which may be involved in SD-induced alteration of the transcriptome of L2/3 IT neurons. We suggest that the transcriptome of L2/3 IT neurons is most impacted by increased sleep pressure among neocortical glutamatergic neuronal subtypes and identify putative molecules involved in such transcriptional alterations.
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来源期刊
Neuroscience Research
Neuroscience Research 医学-神经科学
CiteScore
5.60
自引率
3.40%
发文量
136
审稿时长
28 days
期刊介绍: The international journal publishing original full-length research articles, short communications, technical notes, and reviews on all aspects of neuroscience Neuroscience Research is an international journal for high quality articles in all branches of neuroscience, from the molecular to the behavioral levels. The journal is published in collaboration with the Japan Neuroscience Society and is open to all contributors in the world.
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