托罗罗孢子虫细胞工厂开发对称的单不饱和三酰甘油,用于生产可可脂当量

IF 1.9 4区 农林科学 Q3 CHEMISTRY, APPLIED
Hang Yang, Shuling Lu, Chaofan Sun, Xiaoxue Liu, Linshang Zhang, Lingzhi Cheong, Yanlan Bi
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引用次数: 0

摘要

本研究对碳源、氮源、Mg2+ 和 pH 值等各种因素进行了研究,以确定使用 Toruloides 生产可可脂当量的最佳条件。生物量、脂质含量和脂质产量的最大值分别为 7.8 克/升、61.0% 和 4.4 克/升。R. toruloides 的油脂主要由 16:0、18:0 和 18:1 组成,其中 18:1 在 sn-2 位置上占主导地位,13C NMR 分析进一步证实了这一点。超高效液相色谱-质谱(UPLC-MS)分析表明,在所生产的油脂中,sn-POP、sn-POS 和 sn-SOS 的总和占到三酰甘油种类的 27.11%,这使得这些三酰甘油可以进一步从其他三酰甘油中分离出来,并用于可可脂的等同物。值得注意的是,可可脂当量的产量达到了 1.13 克/升,证明了通过发酵生产 CBE 的科学可行性。这些研究结果表明,发酵法生产可可脂当量前景广阔。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Development of symmetric monounsaturated triacylglycerols by Rhodosporidium toruloides cell factory for cocoa butter equivalent

Development of symmetric monounsaturated triacylglycerols by Rhodosporidium toruloides cell factory for cocoa butter equivalent

Development of symmetric monounsaturated triacylglycerols by Rhodosporidium toruloides cell factory for cocoa butter equivalent

In the present study, various factors including carbon source, nitrogen source, Mg2+, and pH value were investigated to determine the optimal conditions for producing cocoa butter equivalent using R. toruloides. The maximum of biomass, lipid content, and lipid production were achieved at levels of 7.8 g/L, 61.0%, and 4.4 g/L, respectively. The oil from R. toruloides mainly consisted of 16:0, 18:0, and 18:1 with 18:1 dominating at the sn-2 position, which was further confirmed by 13C NMR analysis. UPLC-MS analysis showed that the sum of sn-POP, sn-POS, and sn-SOS accounted for up to 27.11% in the triacylglycerol species of the oil produced, which allows those triacylglycerols to be further separated and isolated from other triacylglycerols and used for cocoa butter equivalent. Notably, the production of cocoa butter equivalent reached 1.13 g/L, demonstrating the scientific feasibility of producing CBE through fermentation. These findings indicate a promising future for the fermentation-based production of cocoa butter equivalent.

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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
95
审稿时长
2.4 months
期刊介绍: The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate. JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of year­to­year, environmental, and/ or cultivar variations through use of appropriate statistical analyses.
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