镧系元素辅助纳米酶在体外诊断中执行光学和磁共振双模态逻辑信号。

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Guixian Shen, Junyao Wang, Pengli Bai* and Zhiyong Wang*, 
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引用次数: 0

摘要

基于铁纳米酶的比色法被广泛应用于体外诊断(IVD)中的生物基质检测,但它也面临着一些局限性。铁纳米酶的最佳催化条件需要强酸环境、高温和其他限制因素;此外,比色结果受光学干扰的影响很大。为解决这些难题,本研究高效制备了掺杂多种过渡元素的铁纳米分子,其中锰修饰的铁纳米分子因其电子传递特性而显示出较高的催化活性。此外,在催化反应中引入镧离子,特别是钕离子,显著提高了羟基自由基的生成效率;重要的是,这种提高延伸到了广泛的 pH 值和温度范围,放大了检测信号。此外,纳米酶的超顺磁性还被用于对底物进行合理的光学和磁共振双模态检测,有效消除了背景光学干扰,确保可靠验证信号的真实性。基于这种磁信号,天然葡萄糖氧化酶与纳米酶的结合使检测灵敏度显著提高了 61.5%,超过了传统比色法的能力。因此,在磁性纳米酶中加入镧系离子可通过双模态信号有效识别生理生物标志物。这不仅保证了更高的灵敏度,还展示了未来应用的巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Lanthanide-Assisted Nanozyme Performs Optical and Magnetic Resonance Dual-Modality Logical Signal for In Vitro Diagnosis

Lanthanide-Assisted Nanozyme Performs Optical and Magnetic Resonance Dual-Modality Logical Signal for In Vitro Diagnosis

Lanthanide-Assisted Nanozyme Performs Optical and Magnetic Resonance Dual-Modality Logical Signal for In Vitro Diagnosis

The iron nanozyme-based colorimetric method, which is widely applied for biosubstrate detection in in vitro diagnosis (IVD), faces some limitations. The optimal catalytic conditions of iron nanozymes necessitate a strong acidic environment, high temperature, and other restrictive factors; additionally, the colorimetric results are highly influenced by optical interferences. To address these challenges, iron nanozymes doped with various transition elements were efficiently prepared in this study, and notably, the manganese-modified one displayed a high catalytic activity owing to its electron transfer property. Furthermore, the introduction of lanthanide ions into the catalytic reactions, specifically the neodymium ion, significantly boosted the generation efficiency of hydroxyl radicals; importantly, this enhancement extended to a wide range of pH levels and temperatures, amplifying the detection signal. Moreover, the nanozyme’s superparamagnetic characteristic was also employed to perform a logical optical and magnetic resonance dual-modality detection for substrates, effectively eliminating background optical interference and ensuring a reliable verification of the signal’s authenticity. Based on this magnetic signal, the integration of natural glucose oxidase with the nanozyme resulted in a notable 61.5% increase in detection sensitivity, surpassing the capabilities of the traditional colorimetric approach. Consequently, the incorporation of lanthanide ions into the magnetic nanozyme enables the effective identification of physiological biomarkers through the dual-modality signal. This not only guarantees enhanced sensitivity but also demonstrates significant potential for future applications.

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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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