乳酸菌、菊粉、多聚葡萄糖和有氧运动通过调节肠道微生物群落和 db/db 小鼠的代谢产物协同改善高血糖症

IF 5.4 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Food & Function Pub Date : 2024-03-06 DOI:10.1039/D3FO04642G
Peifan Li, Tong Tong, Xinyu Shao, Yan Han, Michael Zhang, Yongli Li, Xue Lv, Hao Li and Zuming Li
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引用次数: 0

摘要

本研究旨在评估乳酸菌(L或H代表低剂量或高剂量)、菊粉(I)、聚葡萄糖(P)结合有氧运动(A)对db/db小鼠肠道微生物群组成和代谢谱的影响。经过 12 周的干预后,与 2 型糖尿病小鼠(MC)相比,LIP 组、LIPA 组和 HIPA 组在高血糖、糖耐量、胰岛素抵抗、炎症反应、短链脂肪酸(SCFAs)水平和血脂方面均有显著改善。治疗后,治疗组的肠道微生物群组成发生了有利的变化,有益菌(如乳杆菌、布劳菌、Akkermansia 和 Faecalibaculum)的数量显著增加,变形杆菌的数量显著减少。代谢组学分析表明,与 MC 组相比,LIP 组和 LIPA 组的 5-羟基吲哚乙酸、3-羟基塞巴酸、单磷酸腺苷(AMP)、黄嘌呤和次黄嘌呤含量明显降低,而 3-酮膦酰丙氨酸、鞘氨醇和鞘氨醇含量则分别明显增加。此外,LIP 和 LIPA 不仅改善了鞘脂代谢和嘌呤代谢途径,还激活了 AMP 激活蛋白激酶,通过增加 SCFAs 的水平来促进 β 氧化。Faecalibaculum、Blautia、Bacteroides 和 Akkermansia 与鞘磷脂、3-ketosphinganine 和鞘氨醇呈正相关,与次黄嘌呤、黄嘌呤和 AMP 呈负相关。Faecalibaculum、Blautia、Bacteroides 和 Akkermansia 可能具有改善鞘脂代谢和嘌呤代谢途径的潜力。这些研究结果表明,乳酸菌、菊粉、聚葡萄糖和有氧运动的协同作用为预防和控制 2 型糖尿病提供了有前途的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The synergism of Lactobacillaceae, inulin, polyglucose, and aerobic exercise ameliorates hyperglycemia by modulating the gut microbiota community and the metabolic profiles in db/db mice†

The synergism of Lactobacillaceae, inulin, polyglucose, and aerobic exercise ameliorates hyperglycemia by modulating the gut microbiota community and the metabolic profiles in db/db mice†

This study aimed to assess the impact of Lactobacillaceae (L or H represents a low or high dose), inulin (I), and polydextrose (P) combined with aerobic exercise (A) on the composition of the gut microbiota and metabolic profiles in db/db mice. After a 12-week intervention, LIP, LIPA, and HIPA groups exhibited significant improvements in hyperglycemia, glucose tolerance, insulin resistance, inflammatory response, and short-chain fatty acid (SCFA) and blood lipid levels compared to type 2 diabetes mice (MC). After treatment, the gut microbiota composition shifted favorably in the treatment groups which significantly increased the abundance of beneficial bacteria, such as Bacteroides, Blautia, Akkermansia, and Faecalibaculum, and significantly decreased the abundance of Proteus. Metabolomics analysis showed that compared to the MC group, the contents of 5-hydroxyindoleacetic acid, 3-hydroxysebacic acid, adenosine monophosphate (AMP), xanthine and hypoxanthine were significantly decreased, while 3-ketosphinganine, sphinganine, and sphingosine were significantly increased in the LIP and LIPA groups, respectively. Additionally, LIP and LIPA not only improved sphingolipid metabolism and purine metabolism pathways but also activated AMP-activated protein kinase to promote β-oxidation by increasing the levels of SCFAs. Faecalibaculum, Blautia, Bacteroides, and Akkermansia exhibited positive correlations with sphingosine, 3-ketosphinganine, and sphinganine, and exhibited negative correlations with hypoxanthine, xanthine and AMP. Faecalibaculum, Blautia, Bacteroides, and Akkermansia may have the potential to improve sphingolipid metabolism and purine metabolism pathways. These findings suggest that the synergism of Lactobacillaceae, inulin, polydextrose, and aerobic exercise provides a promising strategy for the prevention and management of type 2 diabetes.

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来源期刊
Food & Function
Food & Function BIOCHEMISTRY & MOLECULAR BIOLOGY-FOOD SCIENCE & TECHNOLOGY
CiteScore
10.10
自引率
6.60%
发文量
957
审稿时长
1.8 months
期刊介绍: Food & Function provides a unique venue for physicists, chemists, biochemists, nutritionists and other food scientists to publish work at the interface of the chemistry, physics and biology of food. The journal focuses on food and the functions of food in relation to health.
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