三种产生丁酸的添加剂对日本刺参生长性能、非特异性免疫力和肠道微生物群影响的比较研究

IF 3 2区 农林科学 Q1 FISHERIES
Longzhen Liu, Cong Wei, Yongmei Li, Mingyang Wang, Yuze Mao, Xiangli Tian
{"title":"三种产生丁酸的添加剂对日本刺参生长性能、非特异性免疫力和肠道微生物群影响的比较研究","authors":"Longzhen Liu,&nbsp;Cong Wei,&nbsp;Yongmei Li,&nbsp;Mingyang Wang,&nbsp;Yuze Mao,&nbsp;Xiangli Tian","doi":"10.1155/2024/6973951","DOIUrl":null,"url":null,"abstract":"<p>The providers of butyric acid, <i>Clostridium butyricum</i> (CB), sodium butyrate (SB), and tributyrin (TB), have been extensively studied as aquafeed additives in recent years. However, no comparative study has been reported on the probiotic effects of CB, SB, and TB as feed additives on sea cucumber (<i>Apostichopus japonicus</i>). A 63-day feeding trial was performed to assess the effects of dietary live cells of <i>C. butyricum</i> (CB group, the basal diet supplemented with 1% CB), sodium butyrate (SB group, the basal diet supplemented with 1% SB), and tributyrin (TB group, the basal diet supplemented with 1% TB) on the growth, non-specific immunity, and intestinal microbiota of <i>A. japonicus</i> with a basal diet group as the control. Results indicated that all three additives considerably increased <i>A. japonicus</i> growth, with dietary CB having the optimal growth-promoting effect. Of the seven non-specific enzyme parameters measured in coelomocytes of <i>A. japonicus</i> (i.e., the activities of phagocytosis, respiratory burst, superoxide dismutase, alkaline phosphatase, acid phosphatase, catalase, and lysozyme), dietary CB, SB, and TB considerably increased the activities of six, five, and six of them, respectively. The immune genes (<i>Aj</i>-p105, <i>Aj</i>-p50, <i>Aj</i>-rel, and <i>Aj</i>-lys) expression in the mid-intestine tissues of <i>A. japonicus</i> was significantly increased by all three additives. The CB group had the highest expression of all four genes. Additionally, the relative expression of <i>Aj</i>-p105, <i>Aj</i>-p50, and <i>Aj</i>-lys genes was significantly up-regulated in the three additive groups after stimulation with inactivated <i>Vibrio splendidus</i>. Dietary CB enhanced the intestinal microbial diversity and richness in <i>A. japonicus</i> while dietary TB decreased them. Meanwhile, dietary CB, SB, and TB significantly enhanced the abundance of Firmicutes, <i>unclassified_f_Rhodobacteraceae</i>, and Proteobacteria, respectively, while dietary CB and SB reduced the abundance of <i>Vibrio</i>. Dietary CB and SB improved the stability of microbial ecosystem in the intestine of <i>A. japonicus</i>. In contrast, dietary TB appeared to have a negative effect on the stability of intestinal microbial ecosystem. All three additives improved the intestinal microbial functions associated with energy production and immunity regulation pathways, which may contribute directly to growth promotion and non-specific immunity enhancement in <i>A. japonicus</i>. Collectively, in terms of enhancing growth and non-specific immunity, as well as improving intestinal microbiota, dietary live cells of <i>C. butyricum</i> exhibited the most effective effects in <i>A. japonicus</i>.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2024 1","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Comparative Study on Effects of Three Butyric Acid-Producing Additives on the Growth Performance, Non-specific Immunity, and Intestinal Microbiota of the Sea Cucumber Apostichopus japonicus\",\"authors\":\"Longzhen Liu,&nbsp;Cong Wei,&nbsp;Yongmei Li,&nbsp;Mingyang Wang,&nbsp;Yuze Mao,&nbsp;Xiangli Tian\",\"doi\":\"10.1155/2024/6973951\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The providers of butyric acid, <i>Clostridium butyricum</i> (CB), sodium butyrate (SB), and tributyrin (TB), have been extensively studied as aquafeed additives in recent years. However, no comparative study has been reported on the probiotic effects of CB, SB, and TB as feed additives on sea cucumber (<i>Apostichopus japonicus</i>). A 63-day feeding trial was performed to assess the effects of dietary live cells of <i>C. butyricum</i> (CB group, the basal diet supplemented with 1% CB), sodium butyrate (SB group, the basal diet supplemented with 1% SB), and tributyrin (TB group, the basal diet supplemented with 1% TB) on the growth, non-specific immunity, and intestinal microbiota of <i>A. japonicus</i> with a basal diet group as the control. Results indicated that all three additives considerably increased <i>A. japonicus</i> growth, with dietary CB having the optimal growth-promoting effect. Of the seven non-specific enzyme parameters measured in coelomocytes of <i>A. japonicus</i> (i.e., the activities of phagocytosis, respiratory burst, superoxide dismutase, alkaline phosphatase, acid phosphatase, catalase, and lysozyme), dietary CB, SB, and TB considerably increased the activities of six, five, and six of them, respectively. The immune genes (<i>Aj</i>-p105, <i>Aj</i>-p50, <i>Aj</i>-rel, and <i>Aj</i>-lys) expression in the mid-intestine tissues of <i>A. japonicus</i> was significantly increased by all three additives. The CB group had the highest expression of all four genes. Additionally, the relative expression of <i>Aj</i>-p105, <i>Aj</i>-p50, and <i>Aj</i>-lys genes was significantly up-regulated in the three additive groups after stimulation with inactivated <i>Vibrio splendidus</i>. Dietary CB enhanced the intestinal microbial diversity and richness in <i>A. japonicus</i> while dietary TB decreased them. Meanwhile, dietary CB, SB, and TB significantly enhanced the abundance of Firmicutes, <i>unclassified_f_Rhodobacteraceae</i>, and Proteobacteria, respectively, while dietary CB and SB reduced the abundance of <i>Vibrio</i>. Dietary CB and SB improved the stability of microbial ecosystem in the intestine of <i>A. japonicus</i>. In contrast, dietary TB appeared to have a negative effect on the stability of intestinal microbial ecosystem. All three additives improved the intestinal microbial functions associated with energy production and immunity regulation pathways, which may contribute directly to growth promotion and non-specific immunity enhancement in <i>A. japonicus</i>. Collectively, in terms of enhancing growth and non-specific immunity, as well as improving intestinal microbiota, dietary live cells of <i>C. butyricum</i> exhibited the most effective effects in <i>A. japonicus</i>.</p>\",\"PeriodicalId\":8225,\"journal\":{\"name\":\"Aquaculture Nutrition\",\"volume\":\"2024 1\",\"pages\":\"\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-02-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquaculture Nutrition\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1155/2024/6973951\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Nutrition","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/2024/6973951","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0

摘要

近年来,人们对丁酸的提供者--丁酸梭菌(CB)、丁酸钠(SB)和三丁氰菊酯(TB)作为水产饲料添加剂进行了广泛研究。然而,目前还没有关于 CB、SB 和 TB 作为饲料添加剂对海参益生菌作用的比较研究报告。研究人员进行了一项为期 63 天的喂养试验,以基础日粮组作为对照,评估日粮中丁酸杆菌活细胞(CB 组,基础日粮中添加 1%的 CB)、丁酸钠(SB 组,基础日粮中添加 1%的 SB)和三丁醇(TB 组,基础日粮中添加 1%的 TB)对日本刺参的生长、非特异性免疫和肠道微生物群的影响。结果表明,这三种添加剂都能显著提高日本鹅的生长速度,其中日粮 CB 的生长促进效果最佳。在日本鳗鲡腹腔细胞中测定的七种非特异性酶参数(即吞噬、呼吸爆发、超氧化物歧化酶、碱性磷酸酶、酸性磷酸酶、过氧化氢酶和溶菌酶的活性)中,日粮 CB、SB 和 TB 分别显著提高了其中六种、五种和六种酶的活性。三种添加剂都显著提高了日本鹅肠中段组织中免疫基因(Aj-p105、Aj-p50、Aj-rel和Aj-lys)的表达。CB 组的四个基因表达量最高。此外,在灭活绚丽弧菌的刺激下,Aj-p105、Aj-p50和Aj-lys基因的相对表达量在三种添加剂组中都明显上调。日粮 CB 提高了日本弧菌肠道微生物的多样性和丰富度,而日粮 TB 则降低了它们。同时,膳食 CB、SB 和 TB 分别显著提高了固缩菌、未分类的罗杆菌科和蛋白菌的丰度,而膳食 CB 和 SB 则降低了弧菌的丰度。日粮 CB 和 SB 提高了日本豚肠道微生物生态系统的稳定性。相比之下,日粮 TB 似乎对肠道微生物生态系统的稳定性有负面影响。三种添加剂都能改善与能量生产和免疫调节途径相关的肠道微生物功能,这可能直接有助于促进日本鹅的生长和提高非特异性免疫力。总之,在促进日本鸦片虫的生长和非特异性免疫以及改善肠道微生物群方面,丁酸杆菌的膳食活细胞对日本鸦片虫的影响最为有效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Comparative Study on Effects of Three Butyric Acid-Producing Additives on the Growth Performance, Non-specific Immunity, and Intestinal Microbiota of the Sea Cucumber Apostichopus japonicus

The providers of butyric acid, Clostridium butyricum (CB), sodium butyrate (SB), and tributyrin (TB), have been extensively studied as aquafeed additives in recent years. However, no comparative study has been reported on the probiotic effects of CB, SB, and TB as feed additives on sea cucumber (Apostichopus japonicus). A 63-day feeding trial was performed to assess the effects of dietary live cells of C. butyricum (CB group, the basal diet supplemented with 1% CB), sodium butyrate (SB group, the basal diet supplemented with 1% SB), and tributyrin (TB group, the basal diet supplemented with 1% TB) on the growth, non-specific immunity, and intestinal microbiota of A. japonicus with a basal diet group as the control. Results indicated that all three additives considerably increased A. japonicus growth, with dietary CB having the optimal growth-promoting effect. Of the seven non-specific enzyme parameters measured in coelomocytes of A. japonicus (i.e., the activities of phagocytosis, respiratory burst, superoxide dismutase, alkaline phosphatase, acid phosphatase, catalase, and lysozyme), dietary CB, SB, and TB considerably increased the activities of six, five, and six of them, respectively. The immune genes (Aj-p105, Aj-p50, Aj-rel, and Aj-lys) expression in the mid-intestine tissues of A. japonicus was significantly increased by all three additives. The CB group had the highest expression of all four genes. Additionally, the relative expression of Aj-p105, Aj-p50, and Aj-lys genes was significantly up-regulated in the three additive groups after stimulation with inactivated Vibrio splendidus. Dietary CB enhanced the intestinal microbial diversity and richness in A. japonicus while dietary TB decreased them. Meanwhile, dietary CB, SB, and TB significantly enhanced the abundance of Firmicutes, unclassified_f_Rhodobacteraceae, and Proteobacteria, respectively, while dietary CB and SB reduced the abundance of Vibrio. Dietary CB and SB improved the stability of microbial ecosystem in the intestine of A. japonicus. In contrast, dietary TB appeared to have a negative effect on the stability of intestinal microbial ecosystem. All three additives improved the intestinal microbial functions associated with energy production and immunity regulation pathways, which may contribute directly to growth promotion and non-specific immunity enhancement in A. japonicus. Collectively, in terms of enhancing growth and non-specific immunity, as well as improving intestinal microbiota, dietary live cells of C. butyricum exhibited the most effective effects in A. japonicus.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Aquaculture Nutrition
Aquaculture Nutrition 农林科学-渔业
CiteScore
7.20
自引率
8.60%
发文量
131
审稿时长
3 months
期刊介绍: Aquaculture Nutrition is published on a bimonthly basis, providing a global perspective on the nutrition of all cultivated aquatic animals. Topics range from extensive aquaculture to laboratory studies of nutritional biochemistry and physiology. The Journal specifically seeks to improve our understanding of the nutrition of aquacultured species through the provision of an international forum for the presentation of reviews and original research papers. Aquaculture Nutrition publishes papers which strive to: increase basic knowledge of the nutrition of aquacultured species and elevate the standards of published aquaculture nutrition research. improve understanding of the relationships between nutrition and the environmental impact of aquaculture. increase understanding of the relationships between nutrition and processing, product quality, and the consumer. help aquaculturalists improve their management and understanding of the complex discipline of nutrition. help the aquaculture feed industry by providing a focus for relevant information, techniques, tools and concepts.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信