肌肉再生标记物 FOXP3 与杜氏肌营养不良症的肌肉损伤有关。

IF 1.4 4区 医学 Q4 CLINICAL NEUROLOGY
Sthephanie Yannin Hernández-de la Cruz , Thania Ordaz-Robles (Methodology, Data curation, Validation, Writing – review & editing) , Marco Antonio Villaldama-Soriano , Cristian Emmanuel Luna-Guzmán , Tomas Almeida-Becerril , Judith Villa-Morales , Alan Cárdenas-Conejo , Eugenia Dolores Ruíz-Cruz , Jorge Maldonado-Hernandez , Mariela Bernabe-Garcia , Lourdes Barbosa-Cortés , Maricela Rodríguez-Cruz
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引用次数: 0

摘要

背景:在杜氏肌营养不良症(DMD)患者中,免疫系统细胞(ISC)合成调节炎症的分子,而炎症是肌肉再生所需的一个过程。这些分子与肌肉损伤之间的关系尚不清楚。在 DMD 中,属于 ISC 的单核细胞受欧米伽-3 脂肪酸(ω-3 LCPUFAs)的调节,但这些脂肪酸是否会影响其他 ISC(如 T 细胞)尚不清楚:我们分析了具有不同下肢肌肉功能的 DMD 患者循环白细胞中肌肉再生标志物(FOXP3 和 AREG)的表达情况,以及ω-3 LCPUFA 是否会调节这些标志物的表达、循环 T 细胞的数量、细胞内细胞因子和疾病进展标志物(CD69 和 CD49d):这项安慰剂对照、双盲、随机研究对 DMD 男孩进行了为期 6 个月的ω-3 LCPUFAs(n = 18)或安慰剂(葵花籽油,n = 13)补充。在补充剂的基线期和第 1、2、3 和 6 个月,对白细胞中的 FOXP3 和 AREG mRNA 表达、T 细胞群的免疫分型、CD49d 和 CD69 标记以及血液样本中的细胞内细胞因子进行了分析:结果:辅助活动患者的 FOXP3 mRNA 水平高于非活动患者(P = 0.015)。FOXP3 mRNA表达与补充ω-3 LCPUFAs后第6个月的Vignos量表评分相关(Rho = -0.526,P = 0.03)。补充ω-3 LCPUFAs六个月后,ω-3 LCPUFAs组的CD49d + CD8 + T细胞数量低于安慰剂组(P = 0.037):结论:FOXP3在肌肉功能最差的DMD患者的循环白细胞中高表达。欧米伽-3 LCPUFAs可能会调节粘附标志物CD49d + CD8 + T细胞的合成,但其对FOXP3的影响尚需进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The muscle regeneration marker FOXP3 is associated with muscle injury in Duchenne muscular dystrophy

Background

In Duchenne muscular dystrophy (DMD), the immune system cells (ISC) synthesize molecules to regulate inflammation, a process needed to regenerate muscle. The relationship between those molecules and the muscle injury is unknown. Monocytes belonging to ISC are regulated by omega-3 fatty acids (ω-3 LCPUFAs) in DMD, but whether those fatty acids influence other ISC like T-cells is unknown.

Objective

We analyzed the expression of the muscle regeneration markers (FOXP3 and AREG) in circulating leukocytes of DMD patients with different lower limb muscle functions and whether ω-3 LCPUFAs regulate the expression of those markers, and the populations of circulating T-cells, their intracellular cytokines, and disease progression (CD69 and CD49d) markers.

Methods

This placebo-controlled, double-blind, randomized study was conducted in DMD boys supplemented with ω-3 LCPUFAs (n = 18) or placebo (sunflower oil, n = 13) for six months. FOXP3 and AREG mRNA expression in leukocytes, immunophenotyping of T-cell populations, CD49d and CD69 markers, and intracellular cytokines in blood samples were analyzed at baseline and months 1, 2, 3, and 6 of supplementation.

Results

Patients with assisted ambulation expressed higher (P = 0.015) FOXP3 mRNA levels than ambulatory patients. The FOXP3 mRNA expression correlated (Rho = -0.526, P = 0.03) with the Vignos scale score at month six of supplementation with ω-3 LCPUFAs. CD49d + CD8 + T-cells population was lower (P = 0.037) in the ω −3 LCPUFAs group than placebo at month six of supplementation.

Conclusion

FOXP3 is highly expressed in circulating leukocytes of DMD patients with the worst muscle function. Omega-3 LCPUFAs might modulate the synthesis of the adhesion marker CD49d + CD8 + T-cells, but their plausible impact on FOXP3 needs more research.

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来源期刊
Brain & Development
Brain & Development 医学-临床神经学
CiteScore
3.60
自引率
0.00%
发文量
153
审稿时长
50 days
期刊介绍: Brain and Development (ISSN 0387-7604) is the Official Journal of the Japanese Society of Child Neurology, and is aimed to promote clinical child neurology and developmental neuroscience. The journal is devoted to publishing Review Articles, Full Length Original Papers, Case Reports and Letters to the Editor in the field of Child Neurology and related sciences. Proceedings of meetings, and professional announcements will be published at the Editor''s discretion. Letters concerning articles published in Brain and Development and other relevant issues are also welcome.
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