放线菌素 X2 对柠檬黄单胞菌亚种的抗菌机理、控制效率和非目标毒性评估

IF 6.2 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Liangliang Gao, Meiling Huang, Qin Xiong, Yan Liang, Lanfang Mi, Yueming Jiang and Jun Zhang*, 
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引用次数: 0

摘要

本研究首次探讨了放线菌素X2(Act-X2)对柠檬黄单胞菌亚种(Xcc)的抗菌机理、控制效率和非目标毒性。在生长曲线试验中,Act-X2 在 0.25 MIC(最低抑制浓度,MIC = 31.25 μg/mL)浓度下几乎完全抑制了 Xcc 的增殖。这种抑制作用是通过增加活性氧(ROS)的产生、阻止生物膜的形成、阻碍细胞内蛋白质的合成以及降低 Xcc 的苹果酸脱氢酶(MDH)和琥珀酸脱氢酶(SDH)的酶活性来实现的。分子对接和定量逆转录酶聚合酶链反应(qRT-PCR)分析结果表明,Act-X2 能稳定地与 RNA 聚合酶、核糖体、苹果酸脱氢酶和琥珀酸脱氢酶结合,抑制它们的活性,从而大幅降低相关基因的表达水平。在预防和治疗柑橘腐烂病方面,Act-X2 的效果远远优于市售农药 Cu2(OH)3Cl。此外,非目标毒性评估表明,Act-X2 对柑橘树没有植物毒性,在接触毒性和土壤毒性试验中对蚯蚓的毒性也很小。这项研究表明,Act-X2 有潜力成为一种有效且环保的抗菌剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antibacterial Mechanism, Control Efficiency, and Nontarget Toxicity Evaluation of Actinomycin X2 against Xanthomonas citri Subsp. citri

Antibacterial Mechanism, Control Efficiency, and Nontarget Toxicity Evaluation of Actinomycin X2 against Xanthomonas citri Subsp. citri

Antibacterial Mechanism, Control Efficiency, and Nontarget Toxicity Evaluation of Actinomycin X2 against Xanthomonas citri Subsp. citri

The present study investigated the antibacterial mechanism, control efficiency, and nontarget toxicity of actinomycin X2 (Act-X2) against Xanthomonas citri subsp. citri (Xcc) for the first time. Act-X2 almost completely inhibited the proliferation of Xcc in the growth curve assay at a concentration of 0.25 MIC (minimum inhibitory concentration, MIC = 31.25 μg/mL). This inhibitory effect was achieved by increasing the production of reactive oxygen species (ROS), blocking the formation of biofilms, obstructing the synthesis of intracellular proteins, and decreasing the enzymatic activities of malate dehydrogenase (MDH) and succinate dehydrogenase (SDH) of Xcc. Molecular docking and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis results indicated that Act-X2 steadily bonded to the RNA polymerase, ribosome, malate dehydrogenase, and succinate dehydrogenase to inhibit their activities, thus drastically reducing the expression levels of related genes. Act-X2 showed far more effectiveness than the commercially available pesticide Cu2(OH)3Cl in the prevention and therapy of citrus canker disease. Furthermore, the nontarget toxicity evaluation demonstrated that Act-X2 was not phytotoxic to citrus trees and exhibited minimal toxicity to earthworms in both contact and soil toxic assays. This study suggests that Act-X2 has the potential as an effective and environmentally friendly antibacterial agent.

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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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