成人 T 细胞白血病/淋巴瘤的蛋白质组分析:基于总蛋白制备和溶液消化方法的生物标记物鉴定策略

IF 2.1 4区 医学 Q3 HEMATOLOGY
Haruka Sudo , Yasuhiro Tonoyama , Emi Ikebe , Hiroo Hasegawa , Hidekatsu Iha , Yo-ichi Ishida
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引用次数: 0

摘要

成人T细胞白血病/淋巴瘤(ATL)由人类T细胞白血病病毒-1型(HTLV-1)感染引起,是一种至今仍难以治愈的恶性血液肿瘤。我们在此建立了一种基于培养 ATL 细胞全细胞蛋白质组学的生物标志物鉴定策略,以寻找新型 ATL 生物标志物。我们采用了四种基于裂解缓冲液和细胞总蛋白质组学添加剂的选定条件组合方案,对 ATL 细胞组(由 11 个细胞系组成)、HTLV-1 感染细胞组(由 6 个细胞系组成)和 HTLV-1 阴性细胞组(由 6 个细胞系组成)进行了差异分析。在分析中,我们发现在 ATL 组中分别有 24 和 27 个蛋白质明显增加(比值≥2.0,p < 0.05)和减少(比值≤0.5,p < 0.05)。之前报道的CCL3和CD30/TNFRSF8被证实是显著增加的蛋白质之一。此外,已鉴定蛋白质与Tax之间的相关性分析表明,RASSF2和GORASP2是新型Tax调控因子的候选者。本文建立的生物标记物鉴定策略有望为ATL和其他疾病的生物标记物鉴定做出贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Proteomic analysis of adult T-cell leukemia/lymphoma: A biomarker identification strategy based on preparation and in-solution digestion methods of total proteins

Adult T-cell leukemia/lymphoma (ATL), caused by human T-cell leukemia virus type-1 (HTLV-1) infection, is a malignant hematologic cancer that remains difficult to cure. We herein established a biomarker identification strategy based on the total cell proteomics of cultured ATL cells to search for novel ATL biomarkers. Four protocols with a combination of selected conditions based on lysis buffers and addition agents for total cell proteomics were used for a differential analysis between the ATL cell group (consisting of 11 cell lines), HTLV-1-infected cell group (consisting of 6 cell lines), and HTLV-1-negative cell group (consisting of 6 cell lines). In the analysis, we identified 24 and 27 proteins that were significantly increased (ratio ≥2.0, p < 0.05) and decreased (ratio ≤ 0.5, p < 0.05), respectively, in the ATL group. Previously reported CCL3 and CD30/TNFRSF8 were confirmed to be among significantly increased proteins. Furthermore, correlation analysis between identified proteins and Tax suggested that RASSF2 and GORASP2 were candidates of novel Tax-regulated factors. The biomarker identification strategy established herein is expected to contribute to the identification of biomarkers for ATL and other diseases.

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来源期刊
Leukemia research
Leukemia research 医学-血液学
CiteScore
4.00
自引率
3.70%
发文量
259
审稿时长
1 months
期刊介绍: Leukemia Research an international journal which brings comprehensive and current information to all health care professionals involved in basic and applied clinical research in hematological malignancies. The editors encourage the submission of articles relevant to hematological malignancies. The Journal scope includes reporting studies of cellular and molecular biology, genetics, immunology, epidemiology, clinical evaluation, and therapy of these diseases.
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