Ming-Yang Bao, Zhe Wang, W. G. Nuez-Ortín, Guiping Zhao, Marleen Dehasque, Z. Du, Meiling Zhang
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Both BA and LPLs supplementations helped to maintain liver health by decreasing the activities of aspartate aminotransferase and alanine aminotransferase in serum. Besides, LPLs supplementation decreased overall lipid deposition in terms of mesenteric fat index and liver lipid content. Furthermore, LPLs supplementation showed unique advantage in improving intestinal barrier, as characterized by the increased villus length and higher expression of the tight junction protein zo-1 expression. LPLs supplementation also increased the alpha diversity index and the abundances of Proteobacteria in the intestinal microbiota which is positively correlated with the abundance of SCFA in the gut. 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引用次数: 0
摘要
溶血磷脂(LPL)和胆汁酸(BA)是水产养殖中常用的乳化剂。本研究调查了饵料中补充溶血磷脂或胆汁酸对大口幼鱼生长性能、脂质沉积和肠道健康的影响。将鱼随机分为三组,一式四份,分别饲喂基础日粮(CON)或含有 300 毫克/千克 LPLs(LPLs)或 300 毫克/千克市售 BA 产品(BA)的日粮,连续饲喂 8 周。结果表明,与对照组相比,补充 LPLs 和 BA 的组增重趋势明显,补充 LPLs 可促进鱼体内蛋白质的沉积。补充 BA 和 LPLs 均能降低血清中天门冬氨酸氨基转移酶和丙氨酸氨基转移酶的活性,有助于维护肝脏健康。此外,从肠系膜脂肪指数和肝脏脂质含量来看,补充 LPLs 可减少整体脂质沉积。此外,补充 LPLs 在改善肠道屏障方面具有独特优势,表现为绒毛长度增加和紧密连接蛋白 zo-1 表达量增加。补充 LPLs 还能提高肠道微生物群的α多样性指数和蛋白菌丰度,而蛋白菌丰度与肠道中 SCFA 的丰度呈正相关。这些发现将促进新型饲料添加剂的应用,特别是为水产养殖业合理选择乳化剂提供依据。
Comparison of Lysophospholipids and Bile Acids on the Growth Performance, Lipid Deposition, and Intestinal Health of Largemouth Bass (Micropterus salmoides)
Lysophospholipids (LPLs) and bile acids (BA) are commonly used as emulsifiers in aquaculture. This study investigated the effects of dietary supplementation of LPLs or BA on the growth performance, lipid deposition, and intestinal health of largemouth juveniles. Fish were randomly allotted into three groups in quadruplicate and fed with a basal diet (CON) or diets containing 300 mg/kg LPLs (LPLs), or 300 mg/kg commercially available BA product (BA) for 8 weeks. The results showed that compared with the control group, LPLs and BA supplemented groups showed a higher weight gain trend, and LPLs supplementation promoted the protein deposition in fish body. Both BA and LPLs supplementations helped to maintain liver health by decreasing the activities of aspartate aminotransferase and alanine aminotransferase in serum. Besides, LPLs supplementation decreased overall lipid deposition in terms of mesenteric fat index and liver lipid content. Furthermore, LPLs supplementation showed unique advantage in improving intestinal barrier, as characterized by the increased villus length and higher expression of the tight junction protein zo-1 expression. LPLs supplementation also increased the alpha diversity index and the abundances of Proteobacteria in the intestinal microbiota which is positively correlated with the abundance of SCFA in the gut. These findings will promote the application of novel feed additives and especially provide a basis for the rational selection of emulsifiers in the aquaculture industry.
期刊介绍:
Aquaculture Nutrition is published on a bimonthly basis, providing a global perspective on the nutrition of all cultivated aquatic animals. Topics range from extensive aquaculture to laboratory studies of nutritional biochemistry and physiology. The Journal specifically seeks to improve our understanding of the nutrition of aquacultured species through the provision of an international forum for the presentation of reviews and original research papers.
Aquaculture Nutrition publishes papers which strive to:
increase basic knowledge of the nutrition of aquacultured species and elevate the standards of published aquaculture nutrition research.
improve understanding of the relationships between nutrition and the environmental impact of aquaculture.
increase understanding of the relationships between nutrition and processing, product quality, and the consumer.
help aquaculturalists improve their management and understanding of the complex discipline of nutrition.
help the aquaculture feed industry by providing a focus for relevant information, techniques, tools and concepts.