基于唾液的高灵敏度生物传感器用于检测乳腺癌生物标记物:HER2 和 CA15-3。

IF 1.5 4区 工程技术 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC
Hsiao-Hsuan Wan, Haochen Zhu, Chao-Ching Chiang, Jian-Sian Li, Fan Ren, Cheng-Tse Tsai, Yu-Te Liao, Dan Neal, Josephine F Esquivel-Upshaw, Stephen J Pearton
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引用次数: 0

摘要

女性乳腺癌的发病率突显了对创新、高效检测方法的迫切需求。本研究通过利用唾液生物标记物来解决这一迫切问题,提供了一种无创、便捷的乳腺癌识别方法。在这项研究中,我们利用市售的一次性检测条(类似于常用的葡萄糖检测条),并对其进行功能化处理,以检测带有 HER2 和 CA15-3 生物标志物的乳腺癌。结果表明,这两种生物标记物的检测限低至 1 fg/ml,远低于传统酶联免疫吸附测定法的 1∼4 ng/ml。通过采用同步双脉冲方法,在传感条的电极和晶体管的漏极上施加 10 个 1.2 毫秒的电压脉冲来放大检测信号,检测信号是这 10 个电压脉冲对应的 10 个数字输出读数的平均值。传感器对 HER2 和 CA15-3 的灵敏度分别达到约 70 分贝和 30 分贝。此外,这项新技术的高效性还体现在它的快速测试时间小于 15 毫秒,且只需 3 μl 唾液样本。这种方法操作简单,将来有可能广泛应用于公众,是乳腺癌早期检测领域的一种变革性工具。这项研究不仅在诊断方法上取得了重要进展,而且有望彻底改变公共卫生实践。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
High sensitivity saliva-based biosensor in detection of breast cancer biomarkers: HER2 and CA15-3.

The prevalence of breast cancer in women underscores the urgent need for innovative and efficient detection methods. This study addresses this imperative by harnessing salivary biomarkers, offering a noninvasive and accessible means of identifying breast cancer. In this study, commercially available disposable based strips similar to the commonly used glucose detection strips were utilized and functionalized to detect breast cancer with biomarkers of HER2 and CA15-3. The results demonstrated limits of detection for these two biomarkers reached as low as 1 fg/ml much lower than those of conventional enzyme-linked immunosorbent assay in the range of 1∼4 ng/ml. By employing a synchronized double-pulse method to apply 10 of 1.2 ms voltage pulses to the electrode of sensing strip and drain electrode of the transistor for amplifying the detected signal, and the detected signal was the average of 10 digital output readings corresponding to those 10 voltage pulses. The sensor sensitivities were achieved approximately 70/dec and 30/dec for HER2 and CA15-3, respectively. Moreover, the efficiency of this novel technique is underscored by its swift testing time of less than 15 ms and its minimal sample requirement of only 3 μl of saliva. The simplicity of operation and the potential for widespread public use in the future position this approach as a transformative tool in the early detection of breast cancer. This research not only provides a crucial advancement in diagnostic methodologies but also holds the promise of revolutionizing public health practices.

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来源期刊
CiteScore
2.70
自引率
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发文量
146
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