Zongzheng Jiang, Dunwei Qian, Zhenye Liang, Sen Wu, Fenglu Han, Chang Xu, Meili Chi, Erchao Li
{"title":"补充膳食必需氨基酸对红爪螯虾幼鱼（Cherax quadricarinatus）生长、消化能力、抗氧化性和肠道健康的评估","authors":"Zongzheng Jiang, Dunwei Qian, Zhenye Liang, Sen Wu, Fenglu Han, Chang Xu, Meili Chi, Erchao Li","doi":"10.1155/2024/8767751","DOIUrl":null,"url":null,"abstract":"<p>The present study was an 8-week feeding trial investigating the effects of lysine and threonine supplementation in vegetable-based diets on growth, antioxidative capacity, and gut microbiota of juvenile redclaw crayfish, <i>Cherax quadricarinatus</i> (initial weight 11.52 ± 0.23 g). The lysine and threonine were supplemented to formulate five isonitrogenous (37%) and isolipidic (9%) diets containing 0% (control), 0.2% lysine (L0.2), 0.2% threonine (T0.2), 0.4% lysine (L0.4), and 0.4% threonine (T0.4), respectively. Compared to the control, weight gain rate (WGR) and specific growth rate (SGR) of <i>C. quadricarinatus</i> significantly increased with increasing dietary lysine and threonine supplementation from 0.2% to 0.4% (<i>P</i> < 0.05). Hepatopancreas trypsin activity significantly increased with increasing levels of lysine and threonine in diets (<i>P</i> < 0.05). However, the pepsin, lipase, and amylase activities were not affected by dietary levels of lysine and threonine (<i>P</i> > 0.05). Compared with the control, crayfish in T0.4 and L0.4 showed significantly higher glutathione peroxidase (GPx) activity (<i>P</i> < 0.05), lower alanine aminotransferase (ALT) activity, and lower malondialdehyde (MDA) content (<i>P</i> < 0.05). Supplementation with 0.4% lysine significantly changed the composition of the gut microbiota (<i>P</i> < 0.05), which showed a significantly increased relative abundance of <i>Proteobacteria</i> and decreased <i>Firmicutes</i>, <i>Actinomycetes</i>, and <i>Pontomyces</i> (<i>P</i> < 0.05). The PICRUSt analysis demonstrated that the abundance of the metabolism and cellular processes pathways in the L0.4 group were markedly decreased compared with the control (<i>P</i> < 0.05). Meanwhile, a tighter interaction of the microbiota community in crayfish was observed in the T0.4 experimental group. In conclusion, these results suggested that dietary supplementation with 0.4% threonine could significantly promote growth and improve microbial health in juvenile <i>C. quadricarinatus</i>.</p>","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":"2024 1","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of Dietary Essential Amino Acid Supplementation on Growth, Digestive Capacity, Antioxidant, and Intestine Health of the Juvenile Redclaw Crayfish, Cherax quadricarinatus\",\"authors\":\"Zongzheng Jiang, Dunwei Qian, Zhenye Liang, Sen Wu, Fenglu Han, Chang Xu, Meili Chi, Erchao Li\",\"doi\":\"10.1155/2024/8767751\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The present study was an 8-week feeding trial investigating the effects of lysine and threonine supplementation in vegetable-based diets on growth, antioxidative capacity, and gut microbiota of juvenile redclaw crayfish, <i>Cherax quadricarinatus</i> (initial weight 11.52 ± 0.23 g). The lysine and threonine were supplemented to formulate five isonitrogenous (37%) and isolipidic (9%) diets containing 0% (control), 0.2% lysine (L0.2), 0.2% threonine (T0.2), 0.4% lysine (L0.4), and 0.4% threonine (T0.4), respectively. Compared to the control, weight gain rate (WGR) and specific growth rate (SGR) of <i>C. quadricarinatus</i> significantly increased with increasing dietary lysine and threonine supplementation from 0.2% to 0.4% (<i>P</i> < 0.05). Hepatopancreas trypsin activity significantly increased with increasing levels of lysine and threonine in diets (<i>P</i> < 0.05). However, the pepsin, lipase, and amylase activities were not affected by dietary levels of lysine and threonine (<i>P</i> > 0.05). Compared with the control, crayfish in T0.4 and L0.4 showed significantly higher glutathione peroxidase (GPx) activity (<i>P</i> < 0.05), lower alanine aminotransferase (ALT) activity, and lower malondialdehyde (MDA) content (<i>P</i> < 0.05). Supplementation with 0.4% lysine significantly changed the composition of the gut microbiota (<i>P</i> < 0.05), which showed a significantly increased relative abundance of <i>Proteobacteria</i> and decreased <i>Firmicutes</i>, <i>Actinomycetes</i>, and <i>Pontomyces</i> (<i>P</i> < 0.05). The PICRUSt analysis demonstrated that the abundance of the metabolism and cellular processes pathways in the L0.4 group were markedly decreased compared with the control (<i>P</i> < 0.05). Meanwhile, a tighter interaction of the microbiota community in crayfish was observed in the T0.4 experimental group. In conclusion, these results suggested that dietary supplementation with 0.4% threonine could significantly promote growth and improve microbial health in juvenile <i>C. quadricarinatus</i>.</p>\",\"PeriodicalId\":8225,\"journal\":{\"name\":\"Aquaculture Nutrition\",\"volume\":\"2024 1\",\"pages\":\"\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-02-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquaculture Nutrition\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1155/2024/8767751\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Nutrition","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/2024/8767751","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
Evaluation of Dietary Essential Amino Acid Supplementation on Growth, Digestive Capacity, Antioxidant, and Intestine Health of the Juvenile Redclaw Crayfish, Cherax quadricarinatus
The present study was an 8-week feeding trial investigating the effects of lysine and threonine supplementation in vegetable-based diets on growth, antioxidative capacity, and gut microbiota of juvenile redclaw crayfish, Cherax quadricarinatus (initial weight 11.52 ± 0.23 g). The lysine and threonine were supplemented to formulate five isonitrogenous (37%) and isolipidic (9%) diets containing 0% (control), 0.2% lysine (L0.2), 0.2% threonine (T0.2), 0.4% lysine (L0.4), and 0.4% threonine (T0.4), respectively. Compared to the control, weight gain rate (WGR) and specific growth rate (SGR) of C. quadricarinatus significantly increased with increasing dietary lysine and threonine supplementation from 0.2% to 0.4% (P < 0.05). Hepatopancreas trypsin activity significantly increased with increasing levels of lysine and threonine in diets (P < 0.05). However, the pepsin, lipase, and amylase activities were not affected by dietary levels of lysine and threonine (P > 0.05). Compared with the control, crayfish in T0.4 and L0.4 showed significantly higher glutathione peroxidase (GPx) activity (P < 0.05), lower alanine aminotransferase (ALT) activity, and lower malondialdehyde (MDA) content (P < 0.05). Supplementation with 0.4% lysine significantly changed the composition of the gut microbiota (P < 0.05), which showed a significantly increased relative abundance of Proteobacteria and decreased Firmicutes, Actinomycetes, and Pontomyces (P < 0.05). The PICRUSt analysis demonstrated that the abundance of the metabolism and cellular processes pathways in the L0.4 group were markedly decreased compared with the control (P < 0.05). Meanwhile, a tighter interaction of the microbiota community in crayfish was observed in the T0.4 experimental group. In conclusion, these results suggested that dietary supplementation with 0.4% threonine could significantly promote growth and improve microbial health in juvenile C. quadricarinatus.
期刊介绍：
Aquaculture Nutrition is published on a bimonthly basis, providing a global perspective on the nutrition of all cultivated aquatic animals. Topics range from extensive aquaculture to laboratory studies of nutritional biochemistry and physiology. The Journal specifically seeks to improve our understanding of the nutrition of aquacultured species through the provision of an international forum for the presentation of reviews and original research papers.
Aquaculture Nutrition publishes papers which strive to:
increase basic knowledge of the nutrition of aquacultured species and elevate the standards of published aquaculture nutrition research.
improve understanding of the relationships between nutrition and the environmental impact of aquaculture.
increase understanding of the relationships between nutrition and processing, product quality, and the consumer.
help aquaculturalists improve their management and understanding of the complex discipline of nutrition.
help the aquaculture feed industry by providing a focus for relevant information, techniques, tools and concepts.
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