{"title":"将 IgM 酶联免疫吸附试验和 56 kDa 基因 PCR 纳入与恙虫病相关的小儿急性脑炎综合征的治疗中。","authors":"Pooja Bhardwaj , Vishal Yadav , Alok Sharma , Shahzadi Gulafshan , Sthita Pragnya Behera , Gaurav Raj Dwivedi , Hirawati Deval , Vijayachari Paluru , Manoj Murhekar , Rajeev Singh","doi":"10.1016/j.idnow.2024.104865","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>To identify the potential target genes for detection of <em>Orientia tsutsugamushi</em> (OT) in pediatric acute encephalitis syndrome (pAES).</p></div><div><h3>Methods</h3><p>DNA was extracted from whole blood of 100 pAES cases having tested positive (n = 41) and negative (n = 59) for scrub typhus (ST) by IgM ELISA. These samples were subjected to standard PCR for 56 kDa, 47 kDa, 16 s rRNA, groEL, traD genes and the newly identified 27 kDa gene.</p></div><div><h3>Results</h3><p>Among the selected gene targets, 56 kDa demonstrated its superiority for OT detection over the other tested genes. The presence of OT was confirmed via PCR targeting 56 kDa gene in 17 out of the 41 (41.4 %) IgM-positive ST AES cases and 38 out of the 59 (64.4 %) ST IgM negative cases. None of the other gene targets were amplified.</p></div><div><h3>Conclusion</h3><p>Integration of serological diagnosis with molecular diagnostics targeting the 56 kDa gene for routine testing of AES patients would facilitate detection of OT in AES endemic regions.</p></div>","PeriodicalId":13539,"journal":{"name":"Infectious diseases now","volume":"54 2","pages":"Article 104865"},"PeriodicalIF":2.9000,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666991924000204/pdfft?md5=3b12ec2a3b2063f17e1d1baf0c57a90d&pid=1-s2.0-S2666991924000204-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Integration of IgM ELISA and 56 kDa gene PCR in management of pediatric acute encephalitis syndrome associated with scrub typhus\",\"authors\":\"Pooja Bhardwaj , Vishal Yadav , Alok Sharma , Shahzadi Gulafshan , Sthita Pragnya Behera , Gaurav Raj Dwivedi , Hirawati Deval , Vijayachari Paluru , Manoj Murhekar , Rajeev Singh\",\"doi\":\"10.1016/j.idnow.2024.104865\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>To identify the potential target genes for detection of <em>Orientia tsutsugamushi</em> (OT) in pediatric acute encephalitis syndrome (pAES).</p></div><div><h3>Methods</h3><p>DNA was extracted from whole blood of 100 pAES cases having tested positive (n = 41) and negative (n = 59) for scrub typhus (ST) by IgM ELISA. These samples were subjected to standard PCR for 56 kDa, 47 kDa, 16 s rRNA, groEL, traD genes and the newly identified 27 kDa gene.</p></div><div><h3>Results</h3><p>Among the selected gene targets, 56 kDa demonstrated its superiority for OT detection over the other tested genes. The presence of OT was confirmed via PCR targeting 56 kDa gene in 17 out of the 41 (41.4 %) IgM-positive ST AES cases and 38 out of the 59 (64.4 %) ST IgM negative cases. None of the other gene targets were amplified.</p></div><div><h3>Conclusion</h3><p>Integration of serological diagnosis with molecular diagnostics targeting the 56 kDa gene for routine testing of AES patients would facilitate detection of OT in AES endemic regions.</p></div>\",\"PeriodicalId\":13539,\"journal\":{\"name\":\"Infectious diseases now\",\"volume\":\"54 2\",\"pages\":\"Article 104865\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-02-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2666991924000204/pdfft?md5=3b12ec2a3b2063f17e1d1baf0c57a90d&pid=1-s2.0-S2666991924000204-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infectious diseases now\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666991924000204\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infectious diseases now","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666991924000204","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Integration of IgM ELISA and 56 kDa gene PCR in management of pediatric acute encephalitis syndrome associated with scrub typhus
Objectives
To identify the potential target genes for detection of Orientia tsutsugamushi (OT) in pediatric acute encephalitis syndrome (pAES).
Methods
DNA was extracted from whole blood of 100 pAES cases having tested positive (n = 41) and negative (n = 59) for scrub typhus (ST) by IgM ELISA. These samples were subjected to standard PCR for 56 kDa, 47 kDa, 16 s rRNA, groEL, traD genes and the newly identified 27 kDa gene.
Results
Among the selected gene targets, 56 kDa demonstrated its superiority for OT detection over the other tested genes. The presence of OT was confirmed via PCR targeting 56 kDa gene in 17 out of the 41 (41.4 %) IgM-positive ST AES cases and 38 out of the 59 (64.4 %) ST IgM negative cases. None of the other gene targets were amplified.
Conclusion
Integration of serological diagnosis with molecular diagnostics targeting the 56 kDa gene for routine testing of AES patients would facilitate detection of OT in AES endemic regions.
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