Xiaolin Wu , Xuanming Lou , Hanzhang Zhou , Joshua Jebaraj Raymond , Leng Gek Kwang , Francesca Yi Teng Ong , Stacy L. Springs , Hanry Yu
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It provides a detailed overview of various Cas proteins for detection, diverse amplification methods to enhance signal, and different digital sample partitioning techniques facilitating absolute quantification. Notably, it explores the latest advancements of CRISPR/Cas and digital assays for food authentication applications. Furthermore, it discusses the potential opportunities and challenges that CRISPR/Cas and dCRISPR present in food authentication.</p></div><div><h3>Key findings and conclusions</h3><p>CRISPR/Cas and dCRISPR are ground breaking technologies for food authentication. When combined with digital sample partitioning, dCRISPR become even more sensitive and accurate than normal CRISPR/Cas-based detection, while also providing absolute quantification. These techniques can detect and quantify food adulterants, identify genetically modified food, distinguish closely-related species, and authenticate clean labels. With improved performance, CRISPR/Cas and dCRISPR have the potential to revolutionize the food supply chain.</p></div>","PeriodicalId":441,"journal":{"name":"Trends in Food Science & Technology","volume":"145 ","pages":"Article 104349"},"PeriodicalIF":15.1000,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection and absolute quantification biosensing tools for food authentication: CRISPR/Cas, digital CRISPR and beyond\",\"authors\":\"Xiaolin Wu , Xuanming Lou , Hanzhang Zhou , Joshua Jebaraj Raymond , Leng Gek Kwang , Francesca Yi Teng Ong , Stacy L. Springs , Hanry Yu\",\"doi\":\"10.1016/j.tifs.2024.104349\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Food authenticity is essential to protect against fraudulent activities and ensure food safety. 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Detection and absolute quantification biosensing tools for food authentication: CRISPR/Cas, digital CRISPR and beyond
Background
Food authenticity is essential to protect against fraudulent activities and ensure food safety. However, traditional authentication methods have limited capabilities due to lengthy testing period, bulky equipment, and low sensitivity and specificity. To address this, CRISPR/Cas and digital CRISPR (dCRISPR) have emerged as potential tools for quick and reliable food authentication.
Scope and approach
This review focuses on the CRISPR/Cas system and absolute quantification technologies, like sample partitioning-based dCRISPR, for food authentication. It provides a detailed overview of various Cas proteins for detection, diverse amplification methods to enhance signal, and different digital sample partitioning techniques facilitating absolute quantification. Notably, it explores the latest advancements of CRISPR/Cas and digital assays for food authentication applications. Furthermore, it discusses the potential opportunities and challenges that CRISPR/Cas and dCRISPR present in food authentication.
Key findings and conclusions
CRISPR/Cas and dCRISPR are ground breaking technologies for food authentication. When combined with digital sample partitioning, dCRISPR become even more sensitive and accurate than normal CRISPR/Cas-based detection, while also providing absolute quantification. These techniques can detect and quantify food adulterants, identify genetically modified food, distinguish closely-related species, and authenticate clean labels. With improved performance, CRISPR/Cas and dCRISPR have the potential to revolutionize the food supply chain.
期刊介绍:
Trends in Food Science & Technology is a prestigious international journal that specializes in peer-reviewed articles covering the latest advancements in technology, food science, and human nutrition. It serves as a bridge between specialized primary journals and general trade magazines, providing readable and scientifically rigorous reviews and commentaries on current research developments and their potential applications in the food industry.
Unlike traditional journals, Trends in Food Science & Technology does not publish original research papers. Instead, it focuses on critical and comprehensive reviews to offer valuable insights for professionals in the field. By bringing together cutting-edge research and industry applications, this journal plays a vital role in disseminating knowledge and facilitating advancements in the food science and technology sector.