Sabrina Diekmann, Stephan Drusch, Monika Brückner-Gühmann
{"title":"葵花籽中富含白蛋白的馏分的界面和乳化特性","authors":"Sabrina Diekmann, Stephan Drusch, Monika Brückner-Gühmann","doi":"10.1002/aocs.12815","DOIUrl":null,"url":null,"abstract":"<p>The interfacial and emulsion properties of mixed albumin-rich fractions extracted from sunflower seeds at pH 3 and pH 5 were analyzed. SDS-PAGE under reducing and non-reducing conditions was used to visualize differences between the protein fractions. The interfacial activity of the albumin-rich fractions was analyzed via drop contour measurement. The viscoelasticity of the protein film the interactions between the protein molecules, and the network forming within the protein film were measured by interfacial shear rheology. Besides being both surface active, albumin-rich fractions extracted at pH 5 were found to form interfacial films that exhibited a greater stability against deformation. In consequence, emulsions prepared with pH 5 extracts showed better properties represented by a smaller oil droplet size and a lower creaming index. The results proof that mixed albumin-rich fractions can stabilize emulsions. Moreover, the presence of co-extracted phenolic compounds seems to be important to generate systems with increased elastic properties of the interfacial film.</p>","PeriodicalId":17182,"journal":{"name":"Journal of the American Oil Chemists Society","volume":"101 10","pages":"997-1012"},"PeriodicalIF":1.9000,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Interfacial and emulsion properties of albumin-rich fractions from sunflower seeds\",\"authors\":\"Sabrina Diekmann, Stephan Drusch, Monika Brückner-Gühmann\",\"doi\":\"10.1002/aocs.12815\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The interfacial and emulsion properties of mixed albumin-rich fractions extracted from sunflower seeds at pH 3 and pH 5 were analyzed. SDS-PAGE under reducing and non-reducing conditions was used to visualize differences between the protein fractions. The interfacial activity of the albumin-rich fractions was analyzed via drop contour measurement. The viscoelasticity of the protein film the interactions between the protein molecules, and the network forming within the protein film were measured by interfacial shear rheology. Besides being both surface active, albumin-rich fractions extracted at pH 5 were found to form interfacial films that exhibited a greater stability against deformation. In consequence, emulsions prepared with pH 5 extracts showed better properties represented by a smaller oil droplet size and a lower creaming index. The results proof that mixed albumin-rich fractions can stabilize emulsions. Moreover, the presence of co-extracted phenolic compounds seems to be important to generate systems with increased elastic properties of the interfacial film.</p>\",\"PeriodicalId\":17182,\"journal\":{\"name\":\"Journal of the American Oil Chemists Society\",\"volume\":\"101 10\",\"pages\":\"997-1012\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-01-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the American Oil Chemists Society\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/aocs.12815\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the American Oil Chemists Society","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/aocs.12815","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Interfacial and emulsion properties of albumin-rich fractions from sunflower seeds
The interfacial and emulsion properties of mixed albumin-rich fractions extracted from sunflower seeds at pH 3 and pH 5 were analyzed. SDS-PAGE under reducing and non-reducing conditions was used to visualize differences between the protein fractions. The interfacial activity of the albumin-rich fractions was analyzed via drop contour measurement. The viscoelasticity of the protein film the interactions between the protein molecules, and the network forming within the protein film were measured by interfacial shear rheology. Besides being both surface active, albumin-rich fractions extracted at pH 5 were found to form interfacial films that exhibited a greater stability against deformation. In consequence, emulsions prepared with pH 5 extracts showed better properties represented by a smaller oil droplet size and a lower creaming index. The results proof that mixed albumin-rich fractions can stabilize emulsions. Moreover, the presence of co-extracted phenolic compounds seems to be important to generate systems with increased elastic properties of the interfacial film.
期刊介绍：
The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate.
JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of yeartoyear, environmental, and/ or cultivar variations through use of appropriate statistical analyses.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
