{"title":"牛乳腺组织中乳腺细胞的表型评估以及纤维化背景下的乳腺炎。","authors":"Cansel Güzin Özgüden-Akkoc, Ayşe Meriç Mutlu, Abdülkadir Keskin, Ezgi Yumuşak, Ahmet Akkoc","doi":"10.1017/S0022029923000651","DOIUrl":null,"url":null,"abstract":"<p><p>This research paper addresses the hypothesis that mast cells (MCs) contribute to the formation of mammary fibrosis. MCs are important immune regulatory and immune modulatory cells that play major roles in the inflammatory process. Since there is no detailed knowledge, this research study aimed to comparatively investigate the presence, localization, and immunophenotypes of MCs in healthy and mastitic mammary tissues. A total of 264 mammary samples were evaluated for the examination of mast cells and fibrosis. The mean mast cell number in both acute and chronic mastitis samples were very significantly higher than the control group <i>P</i> < 0.001). A 7.9-fold increase in the number of mast cells was found when the chronic mastitis group was compared with the control (healthy) group. Immunohistochemistry revealed presence of all three immune phenotypes in control and mastitic mammary samples (tryptase + (MC<sub>T</sub>), chymase + (MC<sub>C</sub>) and both chymase and tryptase + (MC<sub>TC</sub>). The mean MC<sub>T</sub>, MC<sub>C</sub>, and MC<sub>TC</sub> numbers in the chronic mastitis group were found to be significantly higher than the control (<i>P</i> < 0.001 for all three phenotypes) but did not differ significantly between control and acute mastitis samples. When the mean numbers of MC<sub>T</sub>, MC<sub>C</sub>, and MC<sub>TC</sub> in the control group and chronic mastitis group were compared, a 10.5, 7.8, and a 4.1-fold increase was observed, respectively. The amount of connective tissue was strongly increased in tissues with chronic mastitis and a 3.01-fold increase was detected compared to the control group. A statistically significant relation was also found between the amount of fibrosis and the increased number of total MCs (<i>P</i> < 0.001).</p>","PeriodicalId":15615,"journal":{"name":"Journal of Dairy Research","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phenotypic evaluation of mast cells in bovine mammary tissue and mastitis in the context of fibrosis.\",\"authors\":\"Cansel Güzin Özgüden-Akkoc, Ayşe Meriç Mutlu, Abdülkadir Keskin, Ezgi Yumuşak, Ahmet Akkoc\",\"doi\":\"10.1017/S0022029923000651\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This research paper addresses the hypothesis that mast cells (MCs) contribute to the formation of mammary fibrosis. MCs are important immune regulatory and immune modulatory cells that play major roles in the inflammatory process. Since there is no detailed knowledge, this research study aimed to comparatively investigate the presence, localization, and immunophenotypes of MCs in healthy and mastitic mammary tissues. A total of 264 mammary samples were evaluated for the examination of mast cells and fibrosis. The mean mast cell number in both acute and chronic mastitis samples were very significantly higher than the control group <i>P</i> < 0.001). A 7.9-fold increase in the number of mast cells was found when the chronic mastitis group was compared with the control (healthy) group. Immunohistochemistry revealed presence of all three immune phenotypes in control and mastitic mammary samples (tryptase + (MC<sub>T</sub>), chymase + (MC<sub>C</sub>) and both chymase and tryptase + (MC<sub>TC</sub>). The mean MC<sub>T</sub>, MC<sub>C</sub>, and MC<sub>TC</sub> numbers in the chronic mastitis group were found to be significantly higher than the control (<i>P</i> < 0.001 for all three phenotypes) but did not differ significantly between control and acute mastitis samples. When the mean numbers of MC<sub>T</sub>, MC<sub>C</sub>, and MC<sub>TC</sub> in the control group and chronic mastitis group were compared, a 10.5, 7.8, and a 4.1-fold increase was observed, respectively. The amount of connective tissue was strongly increased in tissues with chronic mastitis and a 3.01-fold increase was detected compared to the control group. A statistically significant relation was also found between the amount of fibrosis and the increased number of total MCs (<i>P</i> < 0.001).</p>\",\"PeriodicalId\":15615,\"journal\":{\"name\":\"Journal of Dairy Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2023-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Dairy Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1017/S0022029923000651\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/8 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Dairy Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1017/S0022029923000651","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/8 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Phenotypic evaluation of mast cells in bovine mammary tissue and mastitis in the context of fibrosis.
This research paper addresses the hypothesis that mast cells (MCs) contribute to the formation of mammary fibrosis. MCs are important immune regulatory and immune modulatory cells that play major roles in the inflammatory process. Since there is no detailed knowledge, this research study aimed to comparatively investigate the presence, localization, and immunophenotypes of MCs in healthy and mastitic mammary tissues. A total of 264 mammary samples were evaluated for the examination of mast cells and fibrosis. The mean mast cell number in both acute and chronic mastitis samples were very significantly higher than the control group P < 0.001). A 7.9-fold increase in the number of mast cells was found when the chronic mastitis group was compared with the control (healthy) group. Immunohistochemistry revealed presence of all three immune phenotypes in control and mastitic mammary samples (tryptase + (MCT), chymase + (MCC) and both chymase and tryptase + (MCTC). The mean MCT, MCC, and MCTC numbers in the chronic mastitis group were found to be significantly higher than the control (P < 0.001 for all three phenotypes) but did not differ significantly between control and acute mastitis samples. When the mean numbers of MCT, MCC, and MCTC in the control group and chronic mastitis group were compared, a 10.5, 7.8, and a 4.1-fold increase was observed, respectively. The amount of connective tissue was strongly increased in tissues with chronic mastitis and a 3.01-fold increase was detected compared to the control group. A statistically significant relation was also found between the amount of fibrosis and the increased number of total MCs (P < 0.001).
期刊介绍：
The Journal of Dairy Research is an international Journal of high-standing that publishes original scientific research on all aspects of the biology, wellbeing and technology of lactating animals and the foods they produce. The Journal’s ability to cover the entire dairy foods chain is a major strength. Cross-disciplinary research is particularly welcomed, as is comparative lactation research in different dairy and non-dairy species and research dealing with consumer health aspects of dairy products. Journal of Dairy Research: an international Journal of the lactation sciences.
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