SSEA3(+)人脐带血单核细胞在缺血性中风模型中的治疗作用。

IF 2.4 4区 医学 Q3 NEUROSCIENCES
Dongjie Xiao , Fang Li , Kun Zhang , Guojun Liu , Yunshan Wang , Hua Liu
{"title":"SSEA3(+)人脐带血单核细胞在缺血性中风模型中的治疗作用。","authors":"Dongjie Xiao ,&nbsp;Fang Li ,&nbsp;Kun Zhang ,&nbsp;Guojun Liu ,&nbsp;Yunshan Wang ,&nbsp;Hua Liu","doi":"10.1016/j.neures.2023.12.006","DOIUrl":null,"url":null,"abstract":"<div><p>Numerous evidences showed that human umbilical cord blood (UCB) mononuclear cells were a promising approach for the therapy of ischemic stroke(IS). The effect of stage-specific embryonic antigen 3 (SSEA3)positive subpopulation in UCB was not investigated in IS. In this study, we isolated SSEA3 positive cells from healthy UCB mononuclear cells, which comprised about 7.01% of the total UCB-mononuclear cells. Flow cytometry analysis revealed that SSEA3(+)UCB cells were almost positive for CD44 and CD45, and negative for CD73, CD90 and CD105. The expression of Oct3/4 in SSEA3(+)UCB cells were higher than that in UCB. SSEA3(+)UCB cells sorted by magnetic cell sorting were intravenously injected into the middle cerebral arterial occlusion(MCAO) rat model. Neurological score showed that SSEA3(+)UCB transplantation group exhibited significant improvements in the functional outcome of MCAO rats than UCB transplantation group. Nissl staining and microtubule association protein-2(MAP2) immunofluorescence staining showed that the SSEA3(+)UCB transplantation group decreased neuronal loss. SSEA3(+)UCB transplantation group reduced neuronal apoptosis, inhibited caspase3 expression, and decreased tumor necrosis factor α(TNF-α). These results indicate that SSEA3 positive cells are a novel subpopulation of UCB cells, which exhibit great potential for the treatment of ischemic stroke.</p></div>","PeriodicalId":19146,"journal":{"name":"Neuroscience Research","volume":"203 ","pages":"Pages 42-50"},"PeriodicalIF":2.4000,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168010223002237/pdfft?md5=ab003d840112aff6aceb8ec050dad612&pid=1-s2.0-S0168010223002237-main.pdf","citationCount":"0","resultStr":"{\"title\":\"The therapeutic role of SSEA3(+) human umbilical cord blood mononuclear cells in ischemic stroke model\",\"authors\":\"Dongjie Xiao ,&nbsp;Fang Li ,&nbsp;Kun Zhang ,&nbsp;Guojun Liu ,&nbsp;Yunshan Wang ,&nbsp;Hua Liu\",\"doi\":\"10.1016/j.neures.2023.12.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Numerous evidences showed that human umbilical cord blood (UCB) mononuclear cells were a promising approach for the therapy of ischemic stroke(IS). The effect of stage-specific embryonic antigen 3 (SSEA3)positive subpopulation in UCB was not investigated in IS. In this study, we isolated SSEA3 positive cells from healthy UCB mononuclear cells, which comprised about 7.01% of the total UCB-mononuclear cells. Flow cytometry analysis revealed that SSEA3(+)UCB cells were almost positive for CD44 and CD45, and negative for CD73, CD90 and CD105. The expression of Oct3/4 in SSEA3(+)UCB cells were higher than that in UCB. SSEA3(+)UCB cells sorted by magnetic cell sorting were intravenously injected into the middle cerebral arterial occlusion(MCAO) rat model. Neurological score showed that SSEA3(+)UCB transplantation group exhibited significant improvements in the functional outcome of MCAO rats than UCB transplantation group. Nissl staining and microtubule association protein-2(MAP2) immunofluorescence staining showed that the SSEA3(+)UCB transplantation group decreased neuronal loss. SSEA3(+)UCB transplantation group reduced neuronal apoptosis, inhibited caspase3 expression, and decreased tumor necrosis factor α(TNF-α). These results indicate that SSEA3 positive cells are a novel subpopulation of UCB cells, which exhibit great potential for the treatment of ischemic stroke.</p></div>\",\"PeriodicalId\":19146,\"journal\":{\"name\":\"Neuroscience Research\",\"volume\":\"203 \",\"pages\":\"Pages 42-50\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2023-12-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0168010223002237/pdfft?md5=ab003d840112aff6aceb8ec050dad612&pid=1-s2.0-S0168010223002237-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Neuroscience Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0168010223002237\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuroscience Research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0168010223002237","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

大量证据表明,人类脐带血(UCB)单核细胞是一种治疗缺血性中风(IS)的有效方法。但尚未研究 UCB 中阶段特异性胚胎抗原 3(SSEA3)阳性亚群对缺血性中风的影响。在本研究中,我们从健康的 UCB 单核细胞中分离出了 SSEA3 阳性细胞,约占 UCB 单核细胞总数的 7.01%。流式细胞术分析显示,SSEA3(+)UCB细胞的CD44和CD45几乎呈阳性,而CD73、CD90和CD105呈阴性。SSEA3(+)UCB 细胞中 Oct3/4 的表达高于 UCB。用磁性细胞分拣技术分拣出的SSEA3(+)UCB细胞静脉注射到大脑中动脉闭塞(MCAO)大鼠模型中。神经系统评分显示,SSEA3(+)UCB移植组比UCB移植组明显改善了MCAO大鼠的功能预后。Nissl染色和微管联合蛋白-2(MAP2)免疫荧光染色显示,SSEA3(+)UCB移植组减少了神经元的丢失。SSEA3(+)UCB移植组减少了神经元凋亡,抑制了caspase3的表达,降低了肿瘤坏死因子α(TNF-α)。这些结果表明,SSEA3阳性细胞是UCB细胞的一个新亚群,在治疗缺血性中风方面具有巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The therapeutic role of SSEA3(+) human umbilical cord blood mononuclear cells in ischemic stroke model

Numerous evidences showed that human umbilical cord blood (UCB) mononuclear cells were a promising approach for the therapy of ischemic stroke(IS). The effect of stage-specific embryonic antigen 3 (SSEA3)positive subpopulation in UCB was not investigated in IS. In this study, we isolated SSEA3 positive cells from healthy UCB mononuclear cells, which comprised about 7.01% of the total UCB-mononuclear cells. Flow cytometry analysis revealed that SSEA3(+)UCB cells were almost positive for CD44 and CD45, and negative for CD73, CD90 and CD105. The expression of Oct3/4 in SSEA3(+)UCB cells were higher than that in UCB. SSEA3(+)UCB cells sorted by magnetic cell sorting were intravenously injected into the middle cerebral arterial occlusion(MCAO) rat model. Neurological score showed that SSEA3(+)UCB transplantation group exhibited significant improvements in the functional outcome of MCAO rats than UCB transplantation group. Nissl staining and microtubule association protein-2(MAP2) immunofluorescence staining showed that the SSEA3(+)UCB transplantation group decreased neuronal loss. SSEA3(+)UCB transplantation group reduced neuronal apoptosis, inhibited caspase3 expression, and decreased tumor necrosis factor α(TNF-α). These results indicate that SSEA3 positive cells are a novel subpopulation of UCB cells, which exhibit great potential for the treatment of ischemic stroke.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Neuroscience Research
Neuroscience Research 医学-神经科学
CiteScore
5.60
自引率
3.40%
发文量
136
审稿时长
28 days
期刊介绍: The international journal publishing original full-length research articles, short communications, technical notes, and reviews on all aspects of neuroscience Neuroscience Research is an international journal for high quality articles in all branches of neuroscience, from the molecular to the behavioral levels. The journal is published in collaboration with the Japan Neuroscience Society and is open to all contributors in the world.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信