静态和临时浸泡系统中的液体培养对簕杜鹃(Bambusa vulgaris Schrad.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Yudith García-Ramírez, Marisol Freire-Seijo, Raúl Barbón Rodríguez, Sinesio Torres Garcia
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引用次数: 0

摘要

摘要本研究的目的是比较两种体外培养方法:静态液体培养和临时浸泡系统(TIS)。对离体栽培植物的芽数、芽长和每芽叶片数等形态生理参数进行了测量。此外,还测定了两种条件下培育出的嫩枝的含水量、总酚含量和木质素含量。研究结果表明,使用 TIS 有利于在 TIS 中生长的嫩枝的繁殖、形态和生物化学。在该系统中,每个外植体的长度、芽数和叶片数都有所增加。同样,酚和木质素含量也达到了最高值。然而,在静态液体培养基中生长的嫩枝含水量增加,出现过度水化现象。木质素和总酚的含量也有所下降。这项研究表明,在 TIS 体外繁殖嫩枝可以成功替代传统的液体培养系统,同时还能保持形态和生化质量,达到重新造林的目的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Comparative assessment between liquid culture in static and temporary immersion systems on multiplication, morpho-physiological and biochemical characteristics of Bambusa vulgaris Schrad. ex Wendl. shoots

Comparative assessment between liquid culture in static and temporary immersion systems on multiplication, morpho-physiological and biochemical characteristics of Bambusa vulgaris Schrad. ex Wendl. shoots

Key message

Shoots of B. vulgaris cultured in TIS showed better morpho-physiological and biochemical characteristics, without hyperhydricity during in vitro multiplication.

Abstract

The aim of this study was to compare two in vitro culture methods: the liquid culture in static and temporary immersion system (TIS) and in terms of shoot multiplication and morpho-physiological response of shoots of Bambusa vulgaris Schrad. ex Wendl. The morpho-physiological parameters such as number of shoots, shoot length and number of leaves per shoot were measured οn the plants cultivated in vitro. In addition, water content, total phenolic content, and lignin content were determined οn the shoots developed under both conditions. The results of this study show that the use of TIS favored the propagation, morphology and biochemistry of the shoots grown in TIS. In this system, the length, number of shoots and leaves per explant increased. Likewise, the highest values for phenolic and lignin content were obtained. However, shoots grown in static liquid culture medium experienced an increase in water content and hyperhydricity. Lignin and total phenolic content also decreased. This study shows that in vitro multiplication of shoots at TIS can be a successful alternative to the conventional liquid culture system, while maintaining morphological and biochemical quality for reforestation purposes.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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