{"title":"静态和临时浸泡系统中的液体培养对簕杜鹃(Bambusa vulgaris Schrad.","authors":"Yudith García-Ramírez, Marisol Freire-Seijo, Raúl Barbón Rodríguez, Sinesio Torres Garcia","doi":"10.1007/s11738-023-03637-1","DOIUrl":null,"url":null,"abstract":"<div><h3>Key message</h3><p>Shoots of <i>B. vulgaris</i> cultured in TIS showed better morpho-physiological and biochemical characteristics, without hyperhydricity during in vitro multiplication.</p><h3>Abstract</h3><p>The aim of this study was to compare two in vitro culture methods: the liquid culture in static and temporary immersion system (TIS) and in terms of shoot multiplication and morpho-physiological response of shoots of <i>Bambusa vulgaris</i> Schrad. ex Wendl. The morpho-physiological parameters such as number of shoots, shoot length and number of leaves per shoot were measured οn the plants cultivated in vitro. In addition, water content, total phenolic content, and lignin content were determined οn the shoots developed under both conditions. The results of this study show that the use of TIS favored the propagation, morphology and biochemistry of the shoots grown in TIS. In this system, the length, number of shoots and leaves per explant increased. Likewise, the highest values for phenolic and lignin content were obtained. However, shoots grown in static liquid culture medium experienced an increase in water content and hyperhydricity. Lignin and total phenolic content also decreased. This study shows that in vitro multiplication of shoots at TIS can be a successful alternative to the conventional liquid culture system, while maintaining morphological and biochemical quality for reforestation purposes.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 1","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparative assessment between liquid culture in static and temporary immersion systems on multiplication, morpho-physiological and biochemical characteristics of Bambusa vulgaris Schrad. ex Wendl. shoots\",\"authors\":\"Yudith García-Ramírez, Marisol Freire-Seijo, Raúl Barbón Rodríguez, Sinesio Torres Garcia\",\"doi\":\"10.1007/s11738-023-03637-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Key message</h3><p>Shoots of <i>B. vulgaris</i> cultured in TIS showed better morpho-physiological and biochemical characteristics, without hyperhydricity during in vitro multiplication.</p><h3>Abstract</h3><p>The aim of this study was to compare two in vitro culture methods: the liquid culture in static and temporary immersion system (TIS) and in terms of shoot multiplication and morpho-physiological response of shoots of <i>Bambusa vulgaris</i> Schrad. ex Wendl. The morpho-physiological parameters such as number of shoots, shoot length and number of leaves per shoot were measured οn the plants cultivated in vitro. In addition, water content, total phenolic content, and lignin content were determined οn the shoots developed under both conditions. The results of this study show that the use of TIS favored the propagation, morphology and biochemistry of the shoots grown in TIS. In this system, the length, number of shoots and leaves per explant increased. Likewise, the highest values for phenolic and lignin content were obtained. However, shoots grown in static liquid culture medium experienced an increase in water content and hyperhydricity. Lignin and total phenolic content also decreased. This study shows that in vitro multiplication of shoots at TIS can be a successful alternative to the conventional liquid culture system, while maintaining morphological and biochemical quality for reforestation purposes.</p></div>\",\"PeriodicalId\":6973,\"journal\":{\"name\":\"Acta Physiologiae Plantarum\",\"volume\":\"46 1\",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2023-12-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Physiologiae Plantarum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11738-023-03637-1\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Physiologiae Plantarum","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s11738-023-03637-1","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Comparative assessment between liquid culture in static and temporary immersion systems on multiplication, morpho-physiological and biochemical characteristics of Bambusa vulgaris Schrad. ex Wendl. shoots
Key message
Shoots of B. vulgaris cultured in TIS showed better morpho-physiological and biochemical characteristics, without hyperhydricity during in vitro multiplication.
Abstract
The aim of this study was to compare two in vitro culture methods: the liquid culture in static and temporary immersion system (TIS) and in terms of shoot multiplication and morpho-physiological response of shoots of Bambusa vulgaris Schrad. ex Wendl. The morpho-physiological parameters such as number of shoots, shoot length and number of leaves per shoot were measured οn the plants cultivated in vitro. In addition, water content, total phenolic content, and lignin content were determined οn the shoots developed under both conditions. The results of this study show that the use of TIS favored the propagation, morphology and biochemistry of the shoots grown in TIS. In this system, the length, number of shoots and leaves per explant increased. Likewise, the highest values for phenolic and lignin content were obtained. However, shoots grown in static liquid culture medium experienced an increase in water content and hyperhydricity. Lignin and total phenolic content also decreased. This study shows that in vitro multiplication of shoots at TIS can be a successful alternative to the conventional liquid culture system, while maintaining morphological and biochemical quality for reforestation purposes.
期刊介绍:
Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry.
The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.