海马副白蛋白和神经元周围网:可能参与大鼠的焦虑样行为

IF 2.4 3区 医学 Q3 NEUROSCIENCES
Hippocampus Pub Date : 2023-12-15 DOI:10.1002/hipo.23595
Zhixin Fan, Xiayu Gong, Hanfang Xu, Yue Qu, Bozhi Li, Lanxin Li, Yuqi Yan, Lili Wu, Can Yan
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引用次数: 0

摘要

兴奋-抑制失衡一直被认为是应激相关精神障碍的一个重要机制。在本研究中,我们对大鼠进行了为期 6 天的电击诱导应激。开阔地试验和高架迷宫试验表明,暴露于 IFS 的大鼠表现出更多的焦虑样行为。免疫荧光显示,IFS大鼠背侧海马CA1区GAD67免疫反应的中间神经元密度降低,而CaMKIIα免疫反应的谷氨酸能神经元密度未见明显变化。我们研究了不同的中间神经元亚型标记物的表达,包括副神经元(parvalbumin,PV)、体节素(somatostatin,SST)和钙视蛋白(calretinin,CR),结果发现在IFS大鼠的背侧CA1区,PV免疫反应的中间神经元密度明显下降。神经元周围网(PNN)是一种专门的细胞外基质结构,主要围绕在PV中间神经元周围。我们使用紫藤凝集素凝集素标记PNN,观察到IFS大鼠CA1中PNN包被PV阳性中间神经元的比例增加。在无PNN的PV阳性中间神经元的体节上,PSD95阳性兴奋性突触点的数量明显高于有PNN包被的PV阳性中间神经元。我们的研究结果表明,IFS对海马GABA能中间神经元的影响可能具有细胞类型特异性。背侧海马CA1区PV表型的缺失可能会导致大鼠焦虑。创伤应激暴露后,CA1 中的 PV-PNN 关系失调可能是观察到的焦虑样行为的神经生物学相关因素之一。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hippocampal parvalbumin and perineuronal nets: Possible involvement in anxiety-like behavior in rats

The excitatory-inhibitory imbalance has been considered an important mechanism underlying stress-related psychiatric disorders. In the present study, rats were exposed to 6 days of inescapable foot shock (IFS) to induce stress. The open field test and elevated plus maze test showed that IFS-exposed rats exhibited increased anxiety-like behavior. Immunofluorescence showed that IFS rats had a decreased density of GAD67-immunoreactive interneurons in the dorsal hippocampal CA1 region, while no significant change in the density of CaMKIIα-immunoreactive glutamatergic neurons was seen. We investigated the expression of different interneuron subtype markers, including parvalbumin (PV), somatostatin (SST), and calretinin (CR), and noted a marked decline in the density of PV-immunoreactive interneurons in the dorsal CA1 region of IFS rats. The perineuronal net (PNN) is a specialized extracellular matrix structure primarily around PV interneurons. We used Wisteria floribunda agglutinin lectin to label the PNNs and observed that IFS rats had an increased proportion of PNN-coated PV-positive interneurons in CA1. The number of PSD95-positive excitatory synaptic puncta on the soma of PNN-free PV-positive interneurons was significantly higher than that of PNN-coated PV-positive interneurons. Our findings suggest that the effect of IFS on the hippocampal GABAergic interneurons could be cell-type-specific. Loss of PV phenotype in the dorsal hippocampal CA1 region may contribute to anxiety in rats. The dysregulated PV-PNN relationship in CA1 after traumatic stress exposure might represent one of the neurobiological correlates of the observed anxiety-like behavior.

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来源期刊
Hippocampus
Hippocampus 医学-神经科学
CiteScore
5.80
自引率
5.70%
发文量
79
审稿时长
3-8 weeks
期刊介绍: Hippocampus provides a forum for the exchange of current information between investigators interested in the neurobiology of the hippocampal formation and related structures. While the relationships of submitted papers to the hippocampal formation will be evaluated liberally, the substance of appropriate papers should deal with the hippocampal formation per se or with the interaction between the hippocampal formation and other brain regions. The scope of Hippocampus is wide: single and multidisciplinary experimental studies from all fields of basic science, theoretical papers, papers dealing with hippocampal preparations as models for understanding the central nervous system, and clinical studies will be considered for publication. The Editor especially encourages the submission of papers that contribute to a functional understanding of the hippocampal formation.
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