枣籽油抗氧化活性的研究

Fan Yang, Liang Bai, Yong-qing Tao
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摘要

红枣籽油由酸枣籽压榨而成,在中国广泛种植,具有保健功效。本文主要介绍了红枣籽油的主要成分及其抗氧化活性。采用气相色谱-质谱法对榨出的油进行分析。采用自由基清除法测定其体外抗氧化活性。采用酿酒酵母模型对其体内抗氧化活性进行测定。气相色谱-质谱分析结果表明,烯类化合物(如d-柠檬烯和α-法尼烯)是枣籽油中含量最丰富的成分。体外和体内实验表明,红枣籽油具有良好的抗氧化能力。枣籽油处理可抵消酵母脂质过氧化和胞内氧化诱导的氧化应激。此外,对氧化还原分子缺失菌株的实验表明,红枣籽油诱导的脂质过氧化作用可能与胞质过氧化氢酶tct1基因编码的过氧化氢酶有关。综上所述,我们目前的数据强调了枣籽油的抗氧化能力。有趣的是,Ctt1活性在枣子油脂质过氧化预防机制中起着至关重要的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study on antioxidant activity of Jujube seed oil
Jujube seed oil is pressed by Ziziphi Spinosae Semen,and widely cultivated in China with healthful. Here, we focused on illustrating the components and antioxidant activity of Jujube seed oil. The pressed oil was analyzed by GC-MS. The in vitro antioxidant activity was tested by free radical scavenging. The in vivo antioxidant activity was tested by using Saccharomyces cerevisiae model. The results of GC-MS showed that alkenes (e.g., d-limonene and α-farnesene) were the richest components of jujube seed oil. The experiments of in vitro and in vivo suggested that jujube seed oil possessed good antioxidant capacity. Treatment with Jujube seed oil counteracted oxidative stress-induced by lipid peroxidation and intracellular oxidation in Saccharomyces cerevisiae. Furthermore, experiments performed on missing strains of redox molecules showed that jujube seed oil-induced prevention against lipid peroxidation might be related to catalase encoded by cytosolic catalase T ctt1 gene. Taken together, our current data highlighted the antioxidant capacity of jujube seed oil. Interestingly the Ctt1 activity is vital in the mechanism associated with lipid peroxidation prevention of jujube seed oil.
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