苯硼酸改性虾青素酯的结构特征及其通过阻断活性氧和维持肠道稳态对 DSS 诱导的溃疡性结肠炎的影响

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness Pub Date : 2024-09-01 DOI:10.26599/FSHW.2022.9250223

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引用次数: 0

摘要

通过将苯硼酸（PBA）接枝到虾青素琥珀酸二酯（AstaD）上，构建了一种新型的活性氧（ROS）反应性虾青素苯硼酸衍生物（AstaD-PBA），并鉴定了其化学结构和理化性质。在脂多糖（LPS）诱导的RAW264.7细胞炎症模型中，AstaD-PBA能有效提高ROS淬灭能力。随后，通过LPS、铜（Cu2+）、高脂饮食和右旋糖酐硫酸钠（DSS）诱导的4种斑马鱼ROS反应性炎症模型研究了AstaD-PBA的生物活性。结果表明，AstaD-PBA 可能具有较高的生物安全性，对 DSS 诱导的溃疡性结肠炎（UC）具有最佳效果。此外，AstaD-PBA 还能显著缓解和治疗 C57BL/6J 小鼠的体重减轻和结肠萎缩，抑制炎症细胞因子，维持微生物群平衡，从而改善 UC。根据 16S rRNA 基因测序数据的 Metastats 分析和 Pearson 相关性 Mantel 检验（P < 0.01），Alistipes 和 Oscillibacter 被认为是 UC 标志菌群。总之，AstaD-PBA有望成为改善UC和维持肠道微生物群平衡的功能性化合物。

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Structural characteristics of phenylboronic acid-modified astaxanthin ester and its effect on DSS-induced ulcerative colitis by blocking reactive oxygen species and maintaining intestinal homeostasis

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Structural characteristics of phenylboronic acid-modified astaxanthin ester and its effect on DSS-induced ulcerative colitis by blocking reactive oxygen species and maintaining intestinal homeostasis

A novel and reactive oxygen species (ROS) responsive astaxanthin phenylboronic acid derivative (AstaD-PBA) was constructed by grafting phenylboronic acid (PBA) onto astaxanthin succinate diester (AstaD), and its chemical structure and physicochemical property were identified. AstaD-PBA could effectively improve the ROS quenching ability in the lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model. Then, the bioactivity of AstaD-PBA was studied by 4 zebrafish ROS-responsive inflammatory models induced by LPS, copper (Cu²⁺), high-fat diet, and dextran sodium sulfate (DSS). The results suggest that AstaD-PBA might have high biosafety and the best effect on ulcerative colitis (UC) induced by DSS. Furtherly, AstaD-PBA significantly alleviated and treated weight loss and colonic shrinkage, inhibited inflammatory cytokines, and maintained microbiota homeostasis to improve UC in C57BL/6J mice. Alistipes and Oscillibacter were expected to be considered UC marker flora according to the Metastats analysis and Pearson correlation Mantel test (P < 0.01) of 16S rRNA gene sequencing data. In conclusion, AstaD-PBA has been promised to be a functional compound to improve UC and maintain intestinal microbiota homeostasis.

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来源期刊

Food Science and Human Wellness Agricultural and Biological Sciences-Food Science

CiteScore

8.30

自引率

5.70%

发文量

审稿时长

28 days

期刊介绍： Food Science and Human Wellness is an international peer-reviewed journal that provides a forum for the dissemination of the latest scientific results in food science, nutriology, immunology and cross-field research. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. By their effort, it has been developed to promote the public awareness on diet, advocate healthy diet, reduce the harm caused by unreasonable dietary habit, and directs healthy food development for food industrial producers.