Yingjun Wu, Xiaoyuan Bu, Xinyu Zhou, Zhilin Sha, Xintong Shi
{"title":"聚亚胺2k (PEI2k)/超顺磁氧化铁(SPIO)纳米颗粒通过靶向c-MET和Ets-1抑制肝癌的发展","authors":"Yingjun Wu, Xiaoyuan Bu, Xinyu Zhou, Zhilin Sha, Xintong Shi","doi":"10.1166/mex.2023.2490","DOIUrl":null,"url":null,"abstract":"This study investigates the efficacy of N-Alkyl-polyethylenimine 2 kDa–stabilized superparamagnetic iron oxide ((PEI2k/SPIO) nanoparticles on hepatocellular carcinoma (HCC) in mice and explored the underlying mechanism. Highly metastatic HCC cells were cultured and mRNA expressions of c-MET and Ets-1 were determined by Reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Cell viability was detected by CCK-8 and apoptosis was assessed by flow cytometry. After establishment of animal model for HCC, the rats were administered PEI2k/SPIO nanoparticles and/or Ets-1 inhibitor through tail vein. Cell apoptosis and proliferation were then assessed by EdU experiment and flow cytometry, and the levels of c-MET, Ets-1, MMP-2 were measured as well. HCC cells presented up-regulated c-MET and down-regulated Ets-1. Treatment with PEI2k/SPIO nanoparticles resulted in decreased in c-MET expression and increased Ets-1 in both cells and animals. The PEI2k/SPIO nanoparticles significantly decreased cell proliferation and suppressed tumor growth, and induced apoptosis. Besides, additional injection of Ets-1 enhanced phosphorylation activity of MMP-2 and alleviated PEI2k/SPIO’s effect on MMP-2 expression. Nanotechnology is known to improve delivery efficiency and hence affect prognosis. This study elucidated that, PEI2k/SPIO nanoparticles suppressed malignant characteristics of HCC cells and tumor growth through down-regulation of c-MET and growth factors and up-regulation of MMP-2 and Ets-1.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"77 1","pages":"0"},"PeriodicalIF":0.7000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Polyethylenimine 2k (PEI2k)/superparamagnetic iron oxide (SPIO) nanoparticle inhibits development of hepatocellular carcinoma through targeting of c-MET and Ets-1\",\"authors\":\"Yingjun Wu, Xiaoyuan Bu, Xinyu Zhou, Zhilin Sha, Xintong Shi\",\"doi\":\"10.1166/mex.2023.2490\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study investigates the efficacy of N-Alkyl-polyethylenimine 2 kDa–stabilized superparamagnetic iron oxide ((PEI2k/SPIO) nanoparticles on hepatocellular carcinoma (HCC) in mice and explored the underlying mechanism. Highly metastatic HCC cells were cultured and mRNA expressions of c-MET and Ets-1 were determined by Reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Cell viability was detected by CCK-8 and apoptosis was assessed by flow cytometry. After establishment of animal model for HCC, the rats were administered PEI2k/SPIO nanoparticles and/or Ets-1 inhibitor through tail vein. Cell apoptosis and proliferation were then assessed by EdU experiment and flow cytometry, and the levels of c-MET, Ets-1, MMP-2 were measured as well. HCC cells presented up-regulated c-MET and down-regulated Ets-1. Treatment with PEI2k/SPIO nanoparticles resulted in decreased in c-MET expression and increased Ets-1 in both cells and animals. The PEI2k/SPIO nanoparticles significantly decreased cell proliferation and suppressed tumor growth, and induced apoptosis. Besides, additional injection of Ets-1 enhanced phosphorylation activity of MMP-2 and alleviated PEI2k/SPIO’s effect on MMP-2 expression. Nanotechnology is known to improve delivery efficiency and hence affect prognosis. This study elucidated that, PEI2k/SPIO nanoparticles suppressed malignant characteristics of HCC cells and tumor growth through down-regulation of c-MET and growth factors and up-regulation of MMP-2 and Ets-1.\",\"PeriodicalId\":18318,\"journal\":{\"name\":\"Materials Express\",\"volume\":\"77 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Materials Express\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1166/mex.2023.2490\",\"RegionNum\":4,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Materials Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Materials Express","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1166/mex.2023.2490","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Materials Science","Score":null,"Total":0}
Polyethylenimine 2k (PEI2k)/superparamagnetic iron oxide (SPIO) nanoparticle inhibits development of hepatocellular carcinoma through targeting of c-MET and Ets-1
This study investigates the efficacy of N-Alkyl-polyethylenimine 2 kDa–stabilized superparamagnetic iron oxide ((PEI2k/SPIO) nanoparticles on hepatocellular carcinoma (HCC) in mice and explored the underlying mechanism. Highly metastatic HCC cells were cultured and mRNA expressions of c-MET and Ets-1 were determined by Reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Cell viability was detected by CCK-8 and apoptosis was assessed by flow cytometry. After establishment of animal model for HCC, the rats were administered PEI2k/SPIO nanoparticles and/or Ets-1 inhibitor through tail vein. Cell apoptosis and proliferation were then assessed by EdU experiment and flow cytometry, and the levels of c-MET, Ets-1, MMP-2 were measured as well. HCC cells presented up-regulated c-MET and down-regulated Ets-1. Treatment with PEI2k/SPIO nanoparticles resulted in decreased in c-MET expression and increased Ets-1 in both cells and animals. The PEI2k/SPIO nanoparticles significantly decreased cell proliferation and suppressed tumor growth, and induced apoptosis. Besides, additional injection of Ets-1 enhanced phosphorylation activity of MMP-2 and alleviated PEI2k/SPIO’s effect on MMP-2 expression. Nanotechnology is known to improve delivery efficiency and hence affect prognosis. This study elucidated that, PEI2k/SPIO nanoparticles suppressed malignant characteristics of HCC cells and tumor growth through down-regulation of c-MET and growth factors and up-regulation of MMP-2 and Ets-1.