牛至水乙醇提取物可能的抗炎特性

IF 1.3 Q4 FOOD SCIENCE & TECHNOLOGY
Armenuhi Moghrovyan, Mikayel Ginovyan, Nikolay Avtandilyan, Lilya Parseghyan, Armen Voskanyan, Naira Sahakyan, Anna Darbinyan
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引用次数: 0

摘要

背景:植物化合物因其镇痛、抗炎、细胞毒性等特性而具有重要的应用价值。本研究旨在研究土茯苓水乙醇提取物的化学成分及其对小鼠体内模型伤害性行为和抗炎特性的影响。方法:采用气相色谱-质谱联用技术和高效液相色谱法测定提取物的主要活性化学成分。采用福尔马林和热板试验评估提取物对伤害反应的影响,采用卡拉胶试验确定提取物的抗炎作用。采用甲基噻唑四氮唑法分析提取物对MCF-7乳腺癌细胞的生长抑制作用。结果:芡实水乙醇提取物中检出多种黄酮类化合物和有机酸。提取物对MCF-7细胞具有较低的细胞毒性,仅1 mg/mL或更高浓度的提取物就能引起相当大的生长抑制。福尔马林和热板试验表明,所研究的提取物(5 mg/kg)对小鼠有一定的致敏作用。此外,卡拉胶试验显示了明显的抗炎特性。结论:野生牛至提取物具有较强的生物活性,是开发药用制剂的理想来源。关键词:伤害性行为,抗炎作用,牛至,黄酮类化合物,化学成分,细胞毒性,体内小鼠模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The possible anti-inflammatory properties of hydro-ethanolic extract of Oregano
Backgrounds: Plant compounds are of great value due to their analgesic, anti-inflammatory, cytotoxic, and other characteristics. This study aimed to examine the chemical composition and impact of the Origanum vulgare hydro-ethanolic extract on nociceptive behavior and anti-inflammatory properties in in vivo mice models.Methods: Gas chromatography-mass spectrometry and high-performance liquid chromatography techniques were employed to determine the major active phytochemicals of the extract. The effects of the extract on nociceptive reactions were evaluated using formalin and hot plate tests, while the anti-inflammatory effect was determined using the Carrageenan test. The methyl-thiazolyl-tetrazolium assay was applied to analyze growth-inhibiting properties of the extract on MCF-7 breast cancer cells.Results: Various flavonoids and organic acids were identified in O. vulgare hydro-ethanolic extract. The extract possessed low cytotoxicity on MCF-7 cells as only 1 mg/mL or higher concentrations of the extract induced considerable growth inhibition. The formalin and hot plate tests demonstrated some sensitizing effects of the investigated extract (5 mg/kg) on mice. Moreover, the Carrageenan test revealed pronounced anti-inflammatory properties of the O. vulgare extract.Conclusion: The potent biological activity of the wild oregano herb extract makes it a promising source for the development of medicinal preparations. Keywords: Nociceptive behavior, anti-inflammatory effect, Oregano, flavonoids, chemical composition, cytotoxicity, in vivo mice models.
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来源期刊
Functional Foods in Health and Disease
Functional Foods in Health and Disease FOOD SCIENCE & TECHNOLOGY-
CiteScore
2.20
自引率
20.00%
发文量
47
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