发酵罗布麻果汁对葡聚糖硫酸钠(DSS)诱导的结肠炎小鼠模型中肠道微生物群的影响

IF 5.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Yunyang Zuo, Haoxiang Xu, Dandan Feng, Yuanyuan Shi, Jiuchang Li, Maoyang Wei, Ling Wang, Yulong Zhang, Ping Hu
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引用次数: 0

摘要

本研究主要探讨了由副酸性乳酸杆菌 SR10-1 发酵的 Rosa roxburghii Tratt 果汁(LAB-RRTJ)对葡聚糖硫酸钠(DSS)诱导的小鼠溃疡性结肠炎肠道微生物群的调节作用。与对照组相比,DSS 诱导降低了小鼠体重、Shannon、Simpson、Chao 1 和 Faith_pd 指数,增加了疾病活动指数(DAI)和白细胞介素 1β (IL-1β)、IL-6、肿瘤坏死因子 α (TNF-α) 和干扰素-γ (IFN-γ)水平;这种诱导还导致门级的变形菌、蛭弧菌和放线菌、属级的有害细菌物种丰富度和 S. sciuri、desulfovibib、S. sciuri、desulfovibib 和 S. sciuri 的相对丰富度增加。在小鼠体内,LAB-RRTJ 在物种水平上增加了变形杆菌、蛭弧菌和放线菌,在属水平上增加了有害细菌物种的丰富度,在种水平上增加了 S.sciuri、desulfovibrio C21_c20、R.gnavus 和 Akkermansia muciniphila 的相对丰富度,在门水平上减少了固着菌,减少了 B. acidifaciens 的相对丰富度。LAB-RRTJ能增加DSS诱导的溃疡性结肠炎(UC)小鼠的体重以及Shannon、Simpson、Chao 1和Faith_pd指数,降低DAI和四种炎症因子的含量,改善DSS诱导的UC小鼠肠道微生物群的失衡。此外,LAB-RRTJ治疗后,小鼠OTU数量增加,α多样性和β多样性得到恢复,与对照组相似。与阳性药物治疗组相比,LAB-RRTJ 在调节结肠炎小鼠肠道微生物群多样性方面有更好的效果。相关分析表明,炎症因子与有害菌呈正相关,而与有益菌呈负相关,这在一些结肠炎小鼠中很常见。综上所述,我们的研究表明,LAB-RRTJ 可通过调节炎症细胞因子和肠道微生物群组成,缓解 DSS 诱导的小鼠结肠炎。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effect of fermented Rosa roxburghii tratt fruit juice on gut microbiota in a dextran sulfate sodium (DSS)-induced colitis mouse model

Effect of fermented Rosa roxburghii tratt fruit juice on gut microbiota in a dextran sulfate sodium (DSS)-induced colitis mouse model

This study mainly investigated the regulatory effect of Rosa roxburghii Tratt fruit juice fermented by Lacticaseibacillus paracasei SR10-1 (LAB-RRTJ) on modulating gut microbiota in dextran sulfate sodium (DSS)-induced ulcerative colitis in mice. Compared to control group, DSS induction decreased body weight of mice, indexes of Shannon, Simpson, Chao 1 and Faith_pd, and increased disease activity index (DAI) and levels of interleukin 1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α) and interferon-γ (IFN-γ); And this induction also led to an increase in Proteobacteria, Verrucomicrobia and Actinobacteria at phylum level, harmful bacterial species richness at genus level, and relative richness of S. sciuri, desulfovibrio C21_c20, R. gnavus and Akkermansia muciniphila at species level, and a decrease in Firmicutes at phylum level and relative richness of B. acidifaciens in mice. LAB-RRTJ increased body weight of mice with DSS induced ulcerative colitis (UC) and indexes of Shannon, Simpson, Chao 1 and Faith_pd, reduced DAI and the content of four inflammatory factors and improved gut microbiota imbalance in DSS induced UC mice. Besides, the number of OTUs increased, alpha diversity and beta diversity were restored and similar to those in mice in the Control group after LAB-RRTJ treatment. Compared with the positive drug treatment group, LAB-RRTJ has a better effect on regulating gut microbiota diversity in colitis mice. Correlation analysis showed that inflammatory factors were positively correlated with harmful bacteria and negatively correlated with beneficial bacteria which commonly found in some colitis mice. Taken together, our study demonstrated that LAB-RRTJ could alleviate DSS-induced colitis in mice through the modulation of inflammatory cytokines and gut microbiota composition.

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来源期刊
Food Science and Human Wellness
Food Science and Human Wellness Agricultural and Biological Sciences-Food Science
CiteScore
8.30
自引率
5.70%
发文量
80
审稿时长
28 days
期刊介绍: Food Science and Human Wellness is an international peer-reviewed journal that provides a forum for the dissemination of the latest scientific results in food science, nutriology, immunology and cross-field research. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. By their effort, it has been developed to promote the public awareness on diet, advocate healthy diet, reduce the harm caused by unreasonable dietary habit, and directs healthy food development for food industrial producers.
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