具有配位键断裂和重连接技术的可重复使用的生物分子悬臂式传感器

TRANSDUCERS 2009 - 2009 International Solid-State Sensors, Actuators and Microsystems Conference Pub Date : 2009-06-21 DOI:10.1109/SENSOR.2009.5285722

Haitao Yu, Jungang Li, Xiaohua Gan, Min Liu, P. Xu, Xinxin Li

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引用次数: 1

摘要

本文报道了一种利用配位键断裂和重联技术在微悬臂表面循环固定和擦除抗体的方法。该传感器可多次重复用于生物分子免疫检测。通过对浓度为2000孢子/ml的炭疽芽孢杆菌(BA)孢子灭活疫苗进行三次传感实验，加载在悬臂表面的靶物使其频移分别减少106 Hz、100 Hz和107 Hz。经过多次传感循环后，生物传感器的检测灵敏度没有下降。

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Repeatedly usable bio-molecule cantilever sensors with a coordination-bond breaking and re-linking technique

This paper reports a method for recycled immobilizing and erasing antibody at micro-cantilever surface by a coordination-bond breaking and re-linking technique. With the new method, the sensor can be re-used to immuno-detect bio-molecules for many times. By our sensing experiment to killed vaccine of Bacillus Anthracis (BA) spores with concentration of 2000 spores/ml for three times, the target loaded on the cantilever surface causes a decrease of its frequency shift by 106 Hz, 100 Hz and 107 Hz, respectively. The biosensor detection sensitivity shows no decrease after multiple sensing cycles.

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TRANSDUCERS 2009 - 2009 International Solid-State Sensors, Actuators and Microsystems Conference

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