用于从革兰氏阳性细菌中自动提取RNA的微芯片

TRANSDUCERS 2009 - 2009 International Solid-State Sensors, Actuators and Microsystems Conference Pub Date : 2009-06-21 DOI:10.1109/SENSOR.2009.5285553

P. Vulto, C. Hermann, P. Zahn, U. Maier, G. Dame, G. Urban

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引用次数: 3

摘要

介绍了一种全自动定量提取革兰氏阳性细菌细胞RNA的芯片实验室。该方法结合了热电裂解和电泳纯化。该芯片能够提取RNA与部署的细胞数量成正比，低至1集落形成单位(CFU)。该芯片的性能证明了来自革兰氏阳性细菌嗜热链球菌(S.thermophilus)的传递信使RNA (tmRNA)。RNA提取持续10.5分钟，RNA收率高于市售提取试剂盒。该方法对低分子量rna的提取效率特别高，在临床诊断和药物开发中具有广泛的应用前景。

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A microchip for automated extraction of RNA from Gram-positive bacteria

A Lab-on-a-Chip for fully automated quantitative extraction of RNA from Gram-positive bacterial cells is presented. The method combines thermo-electric lysis with electrophoretic purification. The chip is capable of RNA extraction proportional to the deployed amount of cells down to 1 colony forming units (CFU). The performance of the chip is demonstrated for transfer-messenger RNA (tmRNA) from the Gram-positive bacterium Streptococcus thermophilus (S.thermophilus). The RNA extraction lasts 10.5 minutes and the RNA yield is higher than for commercially available extraction kits. The extraction is particularly efficient for low molecular weight RNAs and may find broad application in clinical diagnostics and drug discovery.

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TRANSDUCERS 2009 - 2009 International Solid-State Sensors, Actuators and Microsystems Conference

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