将DNA注射到具有微机械穿孔结构的动植物组织中

Proceedings IEEE Micro Electro Mechanical Systems. 1995 Pub Date : 1995-01-29 DOI:10.1109/MEMSYS.1995.472544

W. Trimmer, P. Ling, C. Chin, P. Orton, R. Gaugler, S. Hashmi, G. Hashmi, B. Brunett, M. Reed

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引用次数: 44

摘要

硅微机械加工已被用于制造将DNA注入细胞的微探针。在硅衬底上蚀刻出非常尖锐的锥体点阵列。将这些点压入细胞培养液中，使生物活性物质能够穿过细胞壁屏障。利用这种微探针，DNA被注射到植物(烟叶)和动物(线虫)细胞中。

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Injection of DNA into plant and animal tissues with micromechanical piercing structures

Silicon micromachining has been used to fabricate microprobes for injecting DNA into cells. Arrays of very sharp pyramidal points are etched on a silicon substrate. Pressing these points into a culture of cells, allows biologically active material to cross the cell wall barrier. Using the microprobes, DNA has been injected into plant (tobacco leaves) and animal (nematodes) cells.

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Proceedings IEEE Micro Electro Mechanical Systems. 1995

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