免疫金纳米粒子结合表面增强拉曼散射法检测蛋白A

2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems Pub Date : 2007-04-23 DOI:10.1109/NEMS.2007.352209

Chi-Chang Lin, Jr-Tzai Chen, Yan-Fu Chen, T. Chang, Hsien-Chang Chang

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引用次数: 0

摘要

一个独特的，敏感的，高度特异性免疫分析系统使用金纳米粒子和表面增强拉曼光谱(SERS)被描述。为了证明新技术的分析能力，将拉曼报告基因(巯基苯甲酸，MBA)与生物特异性抗蛋白A共同固定在金纳米颗粒(AuNP)上。利用结合电磁场和化学增强的抗蛋白A-AuNP-MBA来利用sers衍生的信号。A蛋白的动态范围为2-3个数量级，检测限为1-10 pg/mL。结果表明，该方法具有快速、高灵敏度、高通量的抗体筛选方法，可用于临床诊断。

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Immunogold Nanoparticle Combing Surface-Enhanced Raman Scattering Method for Protein A Detection

An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and surface-enhanced Raman spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (mercaptobenzoic, MBA) that is coimmobilized with biospeciflc anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-MBA, which combing both electromagnetic field and chemical enhancement, was used to exploits the SERS-derived signal. A dynamic range of 2-3 orders of magnitude and 1-10 pg/mL of detection limitation of protein A were achieved. The results indicated this new technique could be used in clinical diagnostic applications with fast, high sensitivity and high-throughput screening of antibodies.

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2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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