一种用于单细胞捕获和阻抗测量的微流体装置

2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems Pub Date : 2007-04-23 DOI:10.1109/NEMS.2007.352117

Min-Haw Wang, Min-Feng Kao, Haw-Juin Liu, Wai-Hong Kan, Y. Hsu, L. Jang

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引用次数: 4

摘要

介绍了一种用于单细胞捕获和阻抗测量的微流控装置。该装置由带有三个微柱的PDMS通道和带有电极的玻璃基板组成。实验表明，微柱成功捕获了人宫颈上皮样癌HeLa细胞，并通过阻抗谱测量了HeLa细胞的阻抗。工作电压范围为0.1 V ~ 1.5 V，扫描频率范围为1khz ~ 100khz。实验结果表明，HeLa电池具有电容性，其电学模型可简化为一个电阻和一个电容并联。该方法具有物理捕获、成本低、易于制作和测量等优点。

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A Microfluidic Device for Capture of Single Cells and Impedance Measurement

A microfluidic device for capture of single cells and impedance measurement is presented. The device consists of a PDMS channel with three micro pillars and a glass substrate with electrodes. The experiments demonstrated that the HeLa cell (human cervical epithelioid carcinoma) was successfully captured by the micro pillars and its impedance was measured by impedance spectroscopy. The range of operation voltage is from 0.1 V to 1.5 V and the scan frequency is from 1 kHz to 100 kHz. According to experimental results, the HeLa cell is capacitive and its electrical model can be simplified to the parallel connection with one resistor and one capacitor. This developed technique for cell impedance analysis possesses advantages of physical capture, low cost, and easy of fabrication and measurement.

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2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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