基于金属微系统支撑的有机薄膜亲和生物传感器

ASDAM '98. Conference Proceedings. Second International Conference on Advanced Semiconductor Devices and Microsystems (Cat. No.98EX172) Pub Date : 1998-10-05 DOI:10.1109/ASDAM.1998.730236

M. Šnejdárková, L. Sokolikova, V. Tvarozek, I. Novotný, T. Hianik

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引用次数: 0

摘要

利用金微系统载体上的蛋白膜(PF)构建人IgE亲和生物传感器。抗原Q-SwaHU/IgE通过戊二醛与半胱胺或半胱胺-牛血清白蛋白(BSA)配合物结合，通过化学吸附固定在薄金载体工作电极上。用电导法检测了IgE与亲和生物传感器的相互作用。在含有BSA的生物传感器电解质中添加IgE导致膜电导降低，而添加BSA导致传感器电导呈对数型增加。在两种情况下，亲和生物传感器的灵敏度均为nM级。讨论了生物传感器的响应机制。

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Affinity biosensors based on thin organic films on a metal microsystem support

Protein films (PF) on gold microsystem support were used for construction affinity biosensor for detecting human IgE. Antigen Q-SwaHU/IgE was bound with cysteamine or with cystramine-bovine serum albumin (BSA) complex by means of glutaraldehyde and immobilized to the thin gold support working electrode by means of chemisorption. The interaction of IgE with affinity biosensor has been detected by means of conductance. Addition of IgE to the electrolyte contained biosensor with BSA resulted in decrease membrane conductance, which that with BSA resulted in increase of sensor conductance in logarithm shape. in both cases the sensitivity of affinity biosensor was nM level. The mechanisms of biosensor response are discussed.

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ASDAM '98. Conference Proceedings. Second International Conference on Advanced Semiconductor Devices and Microsystems (Cat. No.98EX172)

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