{"title":"弹性微柱阵列对MDCK细胞力学的研究","authors":"Chien-Wen Wang, Wei-Ren Chen, Ching-Chou Wu, Hsien-Chang Chang","doi":"10.1109/NEMS.2007.352215","DOIUrl":null,"url":null,"abstract":"The cellular force is one of the most important parameters of cellular physiological behavior and mechanical interactions between the cell and its environment which could regulate the cell migration, contractility and gene expression. In order to evidence the characteristics of the physiology and cellular mechanics of the epithelial cells, therefore, we use the micro-pillars arrays (MPAs) to measure the traction force of the Madin-Darby canine kidney (MDCK) cells. When MDCK cells adhere on the different geometry of MPAs, the cell will pull MPAs and cause the pillars displacement due to the traction force. Furthermore, the cellular image and the displacement of the pillars could be analyzed by optic microscope system, and could be evaluated the mechanical interactions between the cells and MPAs. Therefore, this method might help us understand the cellular mechanics and physiological phenomenon between the cells and underlying substrates.","PeriodicalId":364039,"journal":{"name":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"29 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2007-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Study on the Cell Mechanics of MDCK Cells by Elastic Micro-pillars Arrays\",\"authors\":\"Chien-Wen Wang, Wei-Ren Chen, Ching-Chou Wu, Hsien-Chang Chang\",\"doi\":\"10.1109/NEMS.2007.352215\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The cellular force is one of the most important parameters of cellular physiological behavior and mechanical interactions between the cell and its environment which could regulate the cell migration, contractility and gene expression. In order to evidence the characteristics of the physiology and cellular mechanics of the epithelial cells, therefore, we use the micro-pillars arrays (MPAs) to measure the traction force of the Madin-Darby canine kidney (MDCK) cells. When MDCK cells adhere on the different geometry of MPAs, the cell will pull MPAs and cause the pillars displacement due to the traction force. Furthermore, the cellular image and the displacement of the pillars could be analyzed by optic microscope system, and could be evaluated the mechanical interactions between the cells and MPAs. Therefore, this method might help us understand the cellular mechanics and physiological phenomenon between the cells and underlying substrates.\",\"PeriodicalId\":364039,\"journal\":{\"name\":\"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems\",\"volume\":\"29 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-04-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/NEMS.2007.352215\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/NEMS.2007.352215","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Study on the Cell Mechanics of MDCK Cells by Elastic Micro-pillars Arrays
The cellular force is one of the most important parameters of cellular physiological behavior and mechanical interactions between the cell and its environment which could regulate the cell migration, contractility and gene expression. In order to evidence the characteristics of the physiology and cellular mechanics of the epithelial cells, therefore, we use the micro-pillars arrays (MPAs) to measure the traction force of the Madin-Darby canine kidney (MDCK) cells. When MDCK cells adhere on the different geometry of MPAs, the cell will pull MPAs and cause the pillars displacement due to the traction force. Furthermore, the cellular image and the displacement of the pillars could be analyzed by optic microscope system, and could be evaluated the mechanical interactions between the cells and MPAs. Therefore, this method might help us understand the cellular mechanics and physiological phenomenon between the cells and underlying substrates.
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