{"title":"NIST单克隆抗体(NISTmAb)标准物质的原子模型结构。","authors":"Christina Bergonzo, D Travis Gallagher","doi":"10.6028/jres.126.012","DOIUrl":null,"url":null,"abstract":"As monoclonal antibodies have become a vital resource in medicine, knowledge of\n their complex molecular structures has increased in importance. Thousands of antibody\n components (Fab and Fc fragments) are described in the Protein Data Bank. Whole\n antibodies have been imaged by electron microscopy methods and in a few cases,\n crystallized. The central hinge lacks a unique stable conformation and its dynamic\n properties are important to antibody function. Monte Carlo and molecular dynamics\n simulations and small-angle scattering methods have been used to analyze the wide range\n of configurations that are accessible to antibodies in solution. In order to support the\n development of antibody-based medicines, the National Institute of Standards and\n Technology (NIST) has released an extensively characterized IgG1κ monoclonal antibody\n (mAb), called the NISTmAb Reference Material 8671. To facilitate modeling of whole\n antibodies we now report the construction of an all-atom 3-D model of the NISTmAb.","PeriodicalId":17039,"journal":{"name":"Journal of Research of the National Institute of Standards and Technology","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9681211/pdf/","citationCount":"4","resultStr":"{\"title\":\"Atomic Model Structure of the NIST Monoclonal Antibody (NISTmAb) Reference Material.\",\"authors\":\"Christina Bergonzo, D Travis Gallagher\",\"doi\":\"10.6028/jres.126.012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"As monoclonal antibodies have become a vital resource in medicine, knowledge of\\n their complex molecular structures has increased in importance. Thousands of antibody\\n components (Fab and Fc fragments) are described in the Protein Data Bank. Whole\\n antibodies have been imaged by electron microscopy methods and in a few cases,\\n crystallized. The central hinge lacks a unique stable conformation and its dynamic\\n properties are important to antibody function. Monte Carlo and molecular dynamics\\n simulations and small-angle scattering methods have been used to analyze the wide range\\n of configurations that are accessible to antibodies in solution. In order to support the\\n development of antibody-based medicines, the National Institute of Standards and\\n Technology (NIST) has released an extensively characterized IgG1κ monoclonal antibody\\n (mAb), called the NISTmAb Reference Material 8671. To facilitate modeling of whole\\n antibodies we now report the construction of an all-atom 3-D model of the NISTmAb.\",\"PeriodicalId\":17039,\"journal\":{\"name\":\"Journal of Research of the National Institute of Standards and Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9681211/pdf/\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Research of the National Institute of Standards and Technology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.6028/jres.126.012\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Research of the National Institute of Standards and Technology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.6028/jres.126.012","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Atomic Model Structure of the NIST Monoclonal Antibody (NISTmAb) Reference Material.
As monoclonal antibodies have become a vital resource in medicine, knowledge of
their complex molecular structures has increased in importance. Thousands of antibody
components (Fab and Fc fragments) are described in the Protein Data Bank. Whole
antibodies have been imaged by electron microscopy methods and in a few cases,
crystallized. The central hinge lacks a unique stable conformation and its dynamic
properties are important to antibody function. Monte Carlo and molecular dynamics
simulations and small-angle scattering methods have been used to analyze the wide range
of configurations that are accessible to antibodies in solution. In order to support the
development of antibody-based medicines, the National Institute of Standards and
Technology (NIST) has released an extensively characterized IgG1κ monoclonal antibody
(mAb), called the NISTmAb Reference Material 8671. To facilitate modeling of whole
antibodies we now report the construction of an all-atom 3-D model of the NISTmAb.
期刊介绍:
The Journal of Research of the National Institute of Standards and Technology is the flagship publication of the National Institute of Standards and Technology. It has been published under various titles and forms since 1904, with its roots as Scientific Papers issued as the Bulletin of the Bureau of Standards.
In 1928, the Scientific Papers were combined with Technologic Papers, which reported results of investigations of material and methods of testing. This new publication was titled the Bureau of Standards Journal of Research.
The Journal of Research of NIST reports NIST research and development in metrology and related fields of physical science, engineering, applied mathematics, statistics, biotechnology, information technology.