Repeated neonatal Needle-pricking stimulation alter neurodevelopment in adolescent rats

IF 1.4 4区医学 Q4 CLINICAL NEUROLOGY

Brain & Development Pub Date : 2023-09-01 DOI:10.1016/j.braindev.2023.04.002

Jing Xu , Youhui Wang , Yu Zuo , Shuai Lv , Dong Li

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引用次数: 1

Abstract

Objective

To explore the repeated pain stimulation in neonatal rats affects their cognitive and memory abilities during puberty, and the proliferation expression of hippocampal neurons.

Methods

Postnatal 1 day (P1) SD rats were randomly divided into two groups, and the skin of the needle group was pricked for seven days consecutively while the skin of the control group was stroked for the same period of time. The rats in both groups were weighed every week, and the Morris water maze experiment was performed from P44 to P49 to test the cognitive and memory abilities of the rats. On P50, the hippocampal tissue was extracted for observation of pathological features and the expressions of Ki-67 and caspase 3 were determined.

Results

With the increase of the days, the body weight of the rats in the needle group increased slightly slower than that of the control group. The escape latency of the needle group was significantly higher than that of the control group in the water maze test at P45 and P48, and the number of times the rats crossing the platform in the needle group was lower than that of the control group. The HE staining of the hippocampal tissue showed that the cells in the needle group were disorganized, with irregular morphology. Under the electron microscope, the structure of neuron cells and organelles is changed in the hippocampal CA1 region of rats. It showed a decrease in the Ki-67 expression and an increase in caspase 3 in the needle group.

Conclusion

Repeated experience of needle-pricking stimulation in neonatal rats can cause cognitive impairment and memory loss in puberty, disrupt hippocampal organization, and diminish neuronal proliferation.

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新生儿反复针刺刺激对青春期大鼠神经发育的影响

目的探讨新生大鼠青春期反复疼痛刺激对其认知记忆能力及海马神经元增殖表达的影响。方法将出生后第1天（P1）SD大鼠随机分为两组，针刺组皮肤连续刺7天，对照组皮肤抚触相同时间。两组大鼠每周称重，从P44到P49进行Morris水迷宫实验，测试大鼠的认知和记忆能力。在P50上，提取海马组织以观察病理特征，并测定Ki-67和caspase 3的表达。结果随着天数的增加，针刺组大鼠的体重增加速度略慢于对照组。在P45和P48的水迷宫试验中，针刺组的逃生潜伏期显著高于对照组，针刺组大鼠穿越平台的次数低于对照组。海马组织HE染色显示，针刺组细胞紊乱，形态不规则。在电子显微镜下，大鼠海马CA1区神经元细胞和细胞器的结构发生了变化。针刺组Ki-67表达下降，caspase 3表达增加。结论新生大鼠反复经历针刺刺激可导致青春期认知障碍和记忆丧失，破坏海马组织，减少神经元增殖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain & Development 医学-临床神经学

CiteScore

3.60

自引率

0.00%

发文量

153

审稿时长

50 days

期刊介绍： Brain and Development (ISSN 0387-7604) is the Official Journal of the Japanese Society of Child Neurology, and is aimed to promote clinical child neurology and developmental neuroscience. The journal is devoted to publishing Review Articles, Full Length Original Papers, Case Reports and Letters to the Editor in the field of Child Neurology and related sciences. Proceedings of meetings, and professional announcements will be published at the Editor''s discretion. Letters concerning articles published in Brain and Development and other relevant issues are also welcome.