Detection of lytic phage infecting flavour-producing strain of Lacticaseibacillus paracasei in the dairy effluents of Kerala.

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Archana Chandran, Athrayil Kalathil Beena, Murugadas Vaiyapuri, Lijo John, S N Rajakumar, Sudheer Babu, Ligimol James, Mundakka Paramban Rahila
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引用次数: 0

Abstract

The performance of the starter culture is a critical factor that decides the quality of fermented milk. Dahi is a fermented milk product popular in India made using a mixed starter culture of lactic acid bacteria comprising acid and flavour producers. The prevalence of bacteriophages in the dairy environment can critically affect the activity of these starter cultures resulting in starter failure. As there is little information available on the occurrence of bacteriophages in the dairy environment of Kerala, this research communication examines the presence of lytic bacteriophages acting against three potential flavour-producing strains of Lacticaseibacillus paracasei (Lc. paracasei). Dairy effluent samples were screened for the presence of phages against the strains of Lc. paracasei by the multiple host enrichment method. Plates showing clearance zone in spot assay were confirmed for the presence of phages by double-layer agar assay. The plaques obtained in the double-layer agar assay were purified for further identification by next-generation sequencing. A bacteriophage infecting one of the three strains of Lc. paracasei was detected by the plaque assay and the blast annotation of the bacteriophage sequence found 86.05% similarity of the phage to Siphoviridae family. The study endorses the need for monitoring phages in the dairy environment to control phage-related starter failure in the state of Kerala.

喀拉拉邦乳业废液中产味副干酪乳杆菌裂解噬菌体的检测。
发酵剂的性能是决定发酵乳质量的关键因素。Dahi是一种发酵乳制品,在印度很受欢迎,使用乳酸菌混合发酵剂培养,包括酸和味生产者。乳业环境中噬菌体的流行可以严重影响这些发酵剂培养物的活性,导致发酵剂失效。由于关于喀拉拉邦乳制品环境中噬菌体发生的信息很少,本研究通讯检查了裂解噬菌体对三种可能产生风味的副干酪乳杆菌(Lc)菌株的作用。paracasei)。对乳业流出物样品进行了噬菌体抗Lc菌株的筛选。Paracasei采用多宿主富集法。通过双层琼脂实验,证实了斑点实验显示的清除区是噬菌体的存在。在双层琼脂实验中获得的斑块被纯化,以便通过下一代测序进一步鉴定。噬菌体感染三株Lc中的一株的噬菌体对噬菌体序列的blast注释发现,该噬菌体与Siphoviridae家族的相似性为86.05%。该研究支持在喀拉拉邦的乳制品环境中监测噬菌体以控制与噬菌体相关的发酵剂失效的必要性。
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来源期刊
Journal of Dairy Research
Journal of Dairy Research 农林科学-奶制品与动物科学
CiteScore
3.80
自引率
4.80%
发文量
117
审稿时长
12-24 weeks
期刊介绍: The Journal of Dairy Research is an international Journal of high-standing that publishes original scientific research on all aspects of the biology, wellbeing and technology of lactating animals and the foods they produce. The Journal’s ability to cover the entire dairy foods chain is a major strength. Cross-disciplinary research is particularly welcomed, as is comparative lactation research in different dairy and non-dairy species and research dealing with consumer health aspects of dairy products. Journal of Dairy Research: an international Journal of the lactation sciences.
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